Influence of the characteristics of plants grown in vitro Bambusa vulgaris var. Schrad vulgaris. ex Wendl. in its acclimatization
Journal Title: Biotecnología Vegetal - Year 2011, Vol 11, Issue 3
Abstract
Acclimatization is a phase of in vitro plants propagation which influences the efficiency of plant regeneration protocols. It is necessary to define the characteristics of a quality plant material to improve acclimatization of Bambusa vulgaris var. vulgaris plants cultured in vitro. The aim of this study was to determine the effect of the characteristics of B. vulgaris var. vulgaris in vitro plants in the acclimatization phase. Then, the influence of the growth development of in vitro plants was considered for their survival during acclimatization. Height and the number of buds and roots per plant were measured after 30 days of planting. Among all the variables, plants height influenced their acclimatization in the greenhouse. Survival rate was significantly different between treatments with plants smaller than 3.0 cm and treatments with plants higher than 3.0 cm. Similar response was obtained after 30 days in the quantification of the number of roots and buds emitted per plant, regardless the presence of roots when planting. Taking into account the results we conclude that the height of the B. vulgaris in vitro plants was the morphological characteristic with major influenced in the survival and development of plants during the acclimatization phase.
Authors and Affiliations
Ortelio Hurtado, Marisol Freire-Seijo, Michel Leiva-Mora, Yudith García-Ramírez
Keywords
Related Articles
- EP ID EP683764
- DOI -
- Views 135
- Downloads 0
In vitro propagation of bamboos
The genus Bambusa represents one of the largest renewable natural resources. Due to its excellent physical and mechanical properties, resistance to insect attacks, beauty and diversity of applications It is a valuable...
Effect of the physical state of the culture medium and the number of subcultures in the in vitro multiplication phase of plants of Bambusa vulgaris var. vulgaris. Schrad. ex Wendl.
Bambusa vulgaris var. vulgaris. Scharde ex Wendl. is a fast growing multi-species which provides benefits to agricultural crops. It has been used in plantations and agroforestry systems. In vitro propagation of this sp...
Study on the effectiveness of RIZOBAC in the process of calllogenesis in potato var. Desiree
This experiment was carried out in a plant biotechnology laboratory at Havana Agronomy University (UNAH), evaluating the effects of different concentrations of bacterial preparations (RIZOBAC) on the obtention of fresh m...
Tissue culture and genetic transformation in Glycine max (L.) Merrill
This work is a brief overview on the background of in vitro tissue culture and genetic transformation of soybean, as well as a brief introduction to the origin, distribution and importance of the crop. It is also aimed...
Subtractive library obtained from a mutant of variety B 4362 with resistance to sugar cane rust
The hypersensitive response is one of the most powerful mechanisms for which the plants resist pathogen attack. Mutations carried out previously on the variety B4362, of sugarcane, originated five mutants that express th...