Influence of the genotype and inoculation density on the differentiation of somatic embryos of Coffea arabica L. cv. Red Caturra and Coffea canephora cv. Robust
Journal Title: Biotecnología Vegetal - Year 2003, Vol 3, Issue 3
Abstract
The conditions were established for the differentiation of somatic embryos from cell suspensions in the genotype Caturra rojo (Coffea arabica) and Robusta (Coffea canephora). Cell suspensions with high embryogenic potentials and stable coefficients of multiplication were used. While studying the density of inoculation, for the phase of differentiation for both varieties, differences appeared in the embryogenic capacity among them, being reached a whole of 556 500 ES.l-1 for the variety Caturra rojo and 298 670 SE.l-1 for the variety Robusta. The biggest number of embryos in torpedo state, were obtained with a density of inoculation of 0.5 gFW.l-1 for the variety Caturra rojo and 5.0 gMF.l-1 for the variety Robusta.
Authors and Affiliations
Raúl Barbón, Elio Jiménez, Alina Capote
Determination of adequate concentrations of 2,4-D and 6-BAP for the induction of morphogenic calli of sweet potato
Tubers from Inivit B 90-1 and Yabú-8, cultivars were put in the laboratories into water bottle and we chose explants of limbs from sprout. Explants were disinfected with Sodium Hypoclorite (Na OCl) with active chlorine (...
Reduction of negative environmental impact generated by residues of plant tissue culture laboratory
The research is based on the activity developed by teaching and research laboratories for biotechnology purposes with an environmental approach to determine potential contamination risk and analyze the residuals genera...
Transient expression of â-glucuronidase in cell suspensions of the banana hybrid cultivar 'FHIA-21' (AAAB)
This work was carried out with the objective of studying parameters that influence transient expression of the enzyme â-glucuronidase in cell suspensions of the plantain hybrid cultivar FHIA-21 (Musa sp. AAAB). Studies...
Total polyphenol content in callus of Theobroma cacao L. ‘UF-650’ clone
Cocoa (Theobroma cacao L.) is of great interest as a source of compounds of natural origin, and polyphenols are those with the highest bioactivity. The objective of this work was to determine the total polyphenol content...
Methodological procedure to calculate the work norms for the laminar flow hood operators in the mass propagation of in vitro plants
The achievement of productive goals in plant mass propagation with the efficiency and efficacy demanded from market competence of in vitro plants nowadays has an unstopped increase of workers productivity, mainly the l...