Intermediate frequency magnetic field at 250.8 kHz does not induce DNA damage or “Adaptive Response” in vitro
Journal Title: Genetics & Applications - Year 2019, Vol 3, Issue 1
Abstract
The aim of this study was to examine whether intermediate frequency (IF) magnetic field (MF) induces genotoxic effects or play a role in the induction of adaptive response after combined exposure to IF MF and ionizing radiation in leukocytes and in adrenocortical carcinoma cell line H295R. Cells were exposed to 250.8 kHz at the magnetic field strength of 80 A/m (equivalent to 100 μT magnetic flux density) for 20 hours, or exposed to IF MF for 20 hours and 24 hours later challenged with ionizing radiation (1.5 Gy X-ray). Evaluation of the DNA damage was performed with alkaline comet assay. Our results showed that there was no significant genotoxic effect of IF MF exposure compared to the controls in both cell types. Furthermore, results did not indicate a statistically significant change in DNA strand breaks in IF MF pre-exposed cells when they were subsequently exposed to 1.5 Gy. Consequently no adaptive response was detected.
Authors and Affiliations
Zsuzsanna Németh, Erzsébet Laczkovich-Szaladják, Annamária Brech, Zsófia Szilágyi, Györgyi Kubinyi, György Thuróczy
Keywords
Related Articles
- EP ID EP592357
- DOI 10.31383/ga.vol3iss1pp42-50
- Views 128
- Downloads 0
Flow cytometry as tool in plant sciences, with emphasis on genome size and ploidy level assessment
Flow cytometry has become the method of choice to measure the DNA content (genome size) in plants. Ease of sample preparation, fast acquisition, and accurate measurements have made the method popular in the domains of pl...
THE APPLICABILITY OF THREE DNA ISOLATION METHODS IN SSR ANALYSIS OF HEXAPLOID PLUM (Prunus domestica L.) CULTIVARS
The main goal of any DNA extraction procedure is to ensure reliable and reproducible results in a simple, fast and inexpensive manner. When it comes to plant tissues, this goal is challenging to achieve due to the presen...
TRIPLOID KARYOTYPES IN PRENATAL DIAGNOSIS AT UNIVERSITY CLINICAL CENTER OF REPUBLIC OF SRPSKA
Triploidy is chromosomal abnormality characterized by the presence of three sets of chromosomes instead of the normal two sets, so the triploid fetus has 69 chromosomes. Within the Department for medical genetics at Univ...
The first DNA barcode record for Rhyacophila bosnica Schmid, 1970 and pairing of adult and larval life stages
Rhyacophila Pictet, 1834 is globally distributed and highly diverse genus of caddisflies (Trichoptera), characterized by numerous regionally endemic species. In the Balkan Peninsula, the highest number of Rhyacophila spe...
ALRATIO - R script for the analysis of relation between the effective and the detected number of alleles
/