INTRALESIONAL INOCULATION OF AUTOLOGOUS NON-CULTURED EPIDERMAL MELANOCYTE SUSPENSION IN VITILIGO- A NOVEL METHOD
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2018, Vol 7, Issue 7
Abstract
BACKGROUND Autologous noncultured epidermal melanocyte suspension (ANEM) inoculation is a valuable option in surgical treatment of Vitiligo. However, this requires special set up and is a tedious procedure. In this study we tried to develop a simple method of direct inoculation of ANEM without producing blisters on vitiligo patches. MATERIALS AND METHODS Totally 50 patients of stable vitiligo not responding to PUVA/PUVASOL therapy were streamlined for this study. Three identical vitiligo sites i.e. test sites, blister sites and control sites were selected. Direct intralesional inoculation of ANEM suspension was done at test sites while at blister sites it was inoculated after aspiration of blister fluid. At control sites, only PBS solution was given. Monthly follow up of the patients for pigmentation was done for three months. Statistical analysis was carried out by Chisquare test and Z test. RESULTS We found pigmentation at 50% of test sites and 70% of blister sites while no pigmentation seen at control sites. Results showed that chances of pigmentation were similar in face and neck region however it was slightly better at blister sites in trunk and limbs. Statistical analysis revealed that the responses between the test and blister sites are significant and also the response of the above method was more consistent on blister sites as compared to Test site. CONCLUSION This study found that the direct inoculation of melanocyte suspension is an effective and simple method for vitiligo patients unresponsive to PUVA therapy. This novel method of direct inoculation of ANEM suspension without producing blister can become a valuable alternative which can be done on outpatient basis.
Authors and Affiliations
Neeraj Srivastava, Mayank Singh, Sudhir Kumar
SEROPREVALENCE OF HSV, HBsAg & HCV IN STD CLINIC ATTENDEES OF A TERTIARY CARE STATE HEADQUARTERS HOSPITAL
BACKGROUND Sexual activity in humans is meant for both procreative and recreational purposes. However, with the discovery of HIV/AIDS in 1981, concerns developed worldwide over unprotected sex. The unprotected sexual act...
AN OBSERVATIONAL STUDY OF TONGUE TIE RELEASE USING BIPOLAR DIATHERMY AT VELLORE DISTRICT IN TAMILNADU
BACKGROUND Ankyloglossia also known as the hypertrophic lingual frenulum, is a condition where there is a limitation to the tongue movement due to the congenitally short frenulum or there is a fibrous band between base o...
A SERO-EPIDEMIOLOGICAL STUDY OF HERPES SIMPLEX VIRUS- 2 ANTIBODIES AMONG PATIENTS WITH SEXUALLY TRANSMITTED INFECTIONS
BACKGROUND Herpes genitalis (HG) is a sexually transmitted infection of great public health importance. HSV-2 is the major cause of the infection in more than 80% of such cases. Many patients with other sexually transmit...
PERIPARTUM CARDIOMYOPATHY: A CASE REPORT
Peripartum cardiomyopathy (PPCM) is a rare form of heart failure with a reported Incidence of 1 per3000 to 1 per 4000 live births and a fatality rate of 20 – 50%.Onset is usually between the last month of pr...
CLINICAL PROFILE OF AETIOLOGICAL AND RISK FACTORS IN YOUNG STROKE
BACKGROUND Stroke constitutes a significant health problem in paediatric population. The reported incidence of childhood stroke is 2-6/100000 children per year in the past 10 years. The impact of childhood strok...