Large-Scale Deletion of Non-Essential Genes Region of Chromosome XV in Saccharomyces Cerevisiae
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 3
Abstract
We firstly applied ‘Latour system’ to Saccharomyces cerevisiae for large-scale deletion, and ~38 kb target region (nt 143376 -181682) of chromosome XV was deleted precisely and efficiently with no foreign sequences left. There are 20 ORFs in the target region, including ars1510, a chromosomal replication origin. The result demonstrates that this method is a useful tool for large-scale chromosomal modification, and for reducing the genome of S. cerevisiae to obtain the minimal cell in synthetic biology. More than that, this method is applicable for further study of analyzing functions and interactions of clusters of genes.
Authors and Affiliations
Xunxun Shi1, Hai Wu, Feijie Li3, Hui Tang, Liping Zhang*
Keywords
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- EP ID EP384574
- DOI 10.9790/3008-1103034650
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