Low Rate of babA2 Genotype among Iranian Helicobacter pylori Clinical Isolates
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 7
Abstract
Introduction: The Blood Group Antigen-Binding Adhesion (babA), Outer Inflammatory Protein (oipA) and Sialic AcidBinding Adhesin (sabA) as outer membrane proteins involved in Helicobacter pylori adherence to gastric mucosa have been suggested to have a role in the pathogenesis. Aim: To investigate the frequency of H. pylori isolates babA2, oipA and sabA genes in Iranian dyspeptic patients. Materials and Methods: DNAs were extracted from H. pylori –positive cultures taken from 100 different dyspeptic patients. Genotyping was performed by Polymerase Chain Reaction (PCR), using the specific primers for babA2, oipA and sabA genes. Chi square test was used to investigate association between variables, p<0.05 was considered statistically significant. Results: All (100%) isolates possessed oipA and sabA genotypes, whereas babA2 was detected in 22% of isolates. There was no significant relationship between presence of genes with clinical outcome. The combined genotype oipA +/ sabA +/ babA2- was correlated with gastritis. The rate of babA2 genotype in our isolates was lower than other Iranian reports. Conclusion: Frequency of babA2 genotype among H. pylori isolates from Southwest of Iran is considerably less than other regions of Iran. Due to heterogeneity of H. pylori strains in different geographic regions, further work will be needed to understand the role of these virulence genes in H. pylori pathogenesis and their possible association with disease outcome.
Authors and Affiliations
Maryam Sohrabi, Reza Khashei, Mahvash Alizadeh, Mohammad-Kazem Hosseini Asl, Mohammad-Ali Nejati, Mahintaj Dara, Abdollah Bazargani
Keywords
Related Articles
- EP ID EP353352
- DOI 10.7860/JCDR/2017/24810.10277
- Views 66
- Downloads 0
Human Epidermal Growth Factor Receptor-3 mRNA Expression as a Prognostic Marker for Invasive Duct Carcinoma not Otherwise Specified
Introduction: Breast cancer is the most common cancer in women and the Erythroblastosis Oncogene B(ErbB) receptor family holds crucial role in its pathogenesis. Human Epidermal Growth Factor Receptor 3 (HER-3) gene over...
Blood Lead Levels in Children of Southwest Iran, Aged 2-6 Years and Associated Factors
Introduction: Lead is one of the toxic metals that can cause several complications in children. Aim: This study was conducted to determine Blood Lead Levels (BLLs) in healthy children and its association with individual...
Superficial Soft Tissue Swellings: Accuracy of Ultrasonography in Diagnosis with Pathological Correlation
ABSTRACT Introduction: Ultrasonography (USG) is a low cost, non invasive, readily available, safe and a patient comfortable modality. With all USG parameters being complimented with the other variables such as demography...
Comparison of Conjunctival Autograft with Suture versus Autograft with Patient’s Own Blood (without Suture) in Pterygium Surgery: A Pilot Study
ABSTRACT Introduction: Suturing is a conventional method for pterygium surgery. Owing to its several disadvantages newer techniques like autologous fibrin glue have been introduced as an alternative method of graft attac...
Outbreak of Dengue Fever in Bundelkhand Region: A Tertiary Care Hospital Study Report
ABSTRACT Introduction: Dengue is most widely spread mosquito-borne viral disease which is a major public health threat globally. The incidence of dengue fever is increasing year after year with morbidity and mortality in...