Low Temperature Stress Induced Changes in Biochemical Parameters, Protein Banding Pattern and Expression of Zat12 and Myb Genes in Rice Seedling 

Abstract

Low temperature stress is one of the main abiotic factors that reduce the productivity of many crops in hilly areas around the world. In this study, rice seedling were exposed to low temperature stress (control, 0°C, -2°C, -4°C and -6°C) for 2 hr to observe its effect on two rice varieties (Basmati-385 and Shaheen Basmati) through ion and proline contents, photosynthetic pigments, total protein content, protein banding pattern and expression of [i]Zat12[/i] and [i]Myb[/i] genes. Resulted showed different patterns of accumulation of Na[sup]+[/sup] K[sup]+[/sup] and Ca[sup]+2[/sup] ions with the decrease in temperature in both varieties. Proline accumulation was gradually increased in both varieties with the decrease in temperature. Photosynthetic pigments (Chlorophyll (Chl) a, b and carotene) were negatively affected by low temperature stress in both varieties, however, carotene content was much affected than Chl a and b. Nonsignificant variation in protein contents was observed at all levels of low temperature, but the effects of low temperature stress on protein banding pattern of Basmti-385 and Shaheen Basmati were different at different treatments. RT-PCR results indicated that ZAT12 was upregulated by short term low temperature stress while OsMYB show slight upregulation at -2°C as compared to the other treatments. This study identified that ZAT12 and OsMYB function as a positive regulator to mediate tolerance of rice seedlings at low temperature stress. 

Authors and Affiliations

Salma Perveen, Kamran Shinwari, Mehmood Jan, Ijaz Malook, Shafiq Rehman, Murad Khan, Muhammad Jamil

Keywords

Related Articles

Agrobacterium-mediated transformation of Nicotiana tabacum by disarmed strain At 699 resulted in considerable raising of growth and development of transgenic plants

The question how long transgenesis invected alterations are demonstrated in a succeeding generations remains of great interest. In this study we describe the development of T[sub]1[/sub]-T[sub]5[/sub] generations of [i]N...

THE OLFACTORY SYSTEM REGULATES ACUTE MOUNTAIN SICKNESS

OBJECTIVE: Hyperventilation is the first response to hypoxia in high altitude (HA). Our study on rats was designed to establish an integrated hypothesis to include hyperventilation, increased activity of hypothalamic- p...

Физиологические отличия симбиотических мутантов гороха с разной степенью нодуляции при инокуляции Rhizobium leguminosarum 

 Изучали морфологические и физиологические особенности развития растений гороха с разной способностью к нодуляции при инфицировании [i]Rhizobium leguminosarum[/i]. Микроскопические исследования выявили отличия при ф...

Изучение влияния высоких концентраций кадмия на интенсивность процессов перекисного окисления липидов и активность антиоксидантных систем этиолированных проростков пшеницы (Triticum aestivum) и ржи (Secale cereale) 

Изучено влияние высоких концентраций CdCl[sub]2[/sub] на интенсивность процессов перекисного  окисления липидов и функционирование антиоксидантных систем этиолированных проростков пшеницы ([i]Triticum aestivum[/i])...

Efficiency of application original preservatives for conservation leukocytes at -40 °C

When frozen leukocytes exposed to harmful factors of the complex, due to their complex cellular structure and high metabolism. Cryopreservatives allow to avoid damages, but most of which are toxic. The aim of the present...

Download PDF file
  • EP ID EP125954
  • DOI -
  • Views 99
  • Downloads 0

How To Cite

Salma Perveen, Kamran Shinwari, Mehmood Jan, Ijaz Malook, Shafiq Rehman, Murad Khan, Muhammad Jamil (2013). Low Temperature Stress Induced Changes in Biochemical Parameters, Protein Banding Pattern and Expression of Zat12 and Myb Genes in Rice Seedling . Журнал стресс-физиологии и биохимии, 9(4), 193-206. https://europub.co.uk./articles/-A-125954