Metabolic profile of extracts obtained from in vitro cultures and plants of field Morinda royoc L., Psidium guajava L. and Morus alba L.
Journal Title: Biotecnología Vegetal - Year 2008, Vol 8, Issue 2
Abstract
The in vitro plant cell and tissue culture is an alternatives to identify new compounds or to increase production of well-known compounds useful for humankind. The aim of this work was to produce biomass of Morinda royoc L, Psidium guajava L var ‘EEA18-40’ and Morus alba L var ‘Criolla’ species. Different regeneration systems were used. Besides, profile of metabolites production in the in vitro cultured biomass and plants from field was determined by means of the combined techniques of liquid chromatography and mass spectrophotometer. In Shoots culture by in vitro culture system was more feasible for the production of biomass with bigger content of dry matter in Morinda royoc, compared with cells and callus system. Yields of extracts were always bigger in the in vitro culture systems than those obtained from field plants. Profiles of compounds detected in plants coming from greenhouses and in vitro cultured shoots were similar in Morinda royoc. Although, in Psidium guajava and Morus alba were different. Results demonstrated that in vitro culture is a potential source to identify and to produce new compounds.
Authors and Affiliations
Alina Capote , Naivy Pérez-Alonso , Anabel Pérez , Raúl Barbón , Enrique Salas , Dirk Wilken , André Gerth , Lutz Müller-Kuhrt , Elio Jiménez
Keywords
Related Articles
- EP ID EP684071
- DOI -
- Views 147
- Downloads 0
Detection and quantification of quitinasa in banana (Musa spp.) leaves inoculated with Mycosphaerella fijiensis
The knowledge of the role of chitinase enzyme in Musa spp- Mycosphaerella fijiensis interaction is still very limited. Therefore, their study is of great interest, mainly in breeding programs. The aim of this study was t...
Effect of inoculum density and immersion time on the production of potato microtubers in temporary immersion systems and field studies
Microtubers can be easily induced in vitro, however so far their small size and low yield have restricted their use as propagules. Temporary immersion systems were used to produce potato microtubers and the effect of c...
Effect of substrates mixtures on conversion phase of Coffea arabica L. cv. ‘Caturra rojo’ plants obtained by somatic embryogenesis
The development of somatic embryogenesis of coffee (Coffea spp.) in liquid culture medium is a viable alternative for the propagation of this specie, but high conversion rates are required. The aim of this paper was to...
Incidence of microbial contaminants in the micropropagation of sugarcane
The incidence of microbial contaminants in Establishment, Multiplication and Rooting stages of the sugarcane micropropagation was determined by visual observation of the culture vessels. The type of microorganism was con...
Callus formation and somatic embryos of Cocos nucifera L. cv. Yellow Indian in semi-solid culture medium
The objective of this work was evaluated the effect of the concentration of 2,4-D and the type of explant in the formation of callus of Cocos nucifera L. cv. Indio Amarillo. The biggest percentages of explants with callu...