METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF ATENOLOL IN TABLET DOSAGE FORM BY RP-HPLC METHOD
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 11
Abstract
A simple, rapid, precise and accurate reverse phase high performance liquid chromatography has been developed and validated for the estimation of Atenolol in tablet formulations. Separation was carried on Waters e 2695 Isocratic HPLC system separation module with EMPOWER 2 software, PDA detector waters 2998 and INERSIL ODS C18 analytical column, (4.6 x 250* 5µm), was operated in isocratic mode using water and acetonitrile (50:50v/v) as mobile phase and at a flow rate of 1.2 ml/min with detection wavelength of 228 nm, by an injection volume of 20 μl and entire separation was carried out at an ambient temperature for the HPLC system. The linearity was found in the range of 50-150 μg/ml and showed a correlation coefficient of 0.9981. The retention time of Atenolol was found to be 1.2. This study concluded that the proposed method was found to be accurate, reproducible, and consistent. The proposed method can be effectively used for the routine analysis of the drug in marketed formulations.
Authors and Affiliations
Sunitha Panthagada , Satyavathi Kancherla , Lavanya Kandiboti , Anil Appapurapu , Subbarao Gutta
Keywords
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