Metody chemicznej ligacji w syntezie peptydów i białek. Część 2
Journal Title: Wiadomości Chemiczne - Year 2017, Vol 71, Issue 9
Abstract
Proteins are synthesized only by living organisms. Today, we are able to receive them by recombinant protein expression in bacterial cells. This technique is very useful and gives satisfactory amount of desirable material but it precludes the possibility of introduction of some chemical modifications that are often obligatory. For this reason, chemical synthesis of longer peptide chains is still important and is the object of scientists attention. Over the last century, notion of peptide synthesis took a new meaning. Nowadays, we know a number of innovative methods and also automated devices which help us to make progress in this area. Nevertheless, the synthesis of longer, more complicated peptide chains and proteins still constitutes a problem. Native chemical ligation (NCL) has facilitated the synthesis of numerous complex peptide and protein targets. Expansion of ligation techniques has allowed the entry of peptides into the world of therapeutic drugs [1]. NCL reactions are carried out in aqueous solution and give good yields. Due to mild conditions, NCL overcomes racemic and solubility problems encountered in classical peptide synthesis using protected fragments. The challenge is to synthesize the C-terminal thioester-containing peptide necessary for the transesterification reaction, which is the first step of linking the peptide fragments [2]. In this review we discuss the evolution, advantages and potential applications of chemical ligation reactions. In the first part of this article we described the utility of native chemical ligation approach to non-cysteine containing peptides. This part details a number of important approaches to the synthesis of peptides bearing a C-terminal thioester. Contemporary applications of these techniques to the total chemical synthesis of proteins are also presented.
Authors and Affiliations
Katarzyna Jędrzejewska, Magdalena Kropidłowska, Ewa Wieczerzak
Keywords
Related Articles
- EP ID EP570922
- DOI -
- Views 139
- Downloads 0
Katalizatory metatezy olefin, modyfikacje liganda NHC
Olefin metathesis is one of the most important synthetic tools, both in organic and olefin chemistry. Olefin metathesis allows to shorten production time of organic compounds and it is characterized by a smaller amount o...
Elektroforeza swobodna z przepływem hydrodynamicznym : podstawy teoretyczne i rozwiązania techniczne
Free flow electrophoresis, or carrier-free electrophoresis, is a technique based upon the principle of mobility of chargeable species in the electric field. However, unlike more widespread gel electrophoresis, free flow...
Nowe metody w badaniach struktur polikryształów
In this chapter, information on good laboratory practice in the field of structural powder diffractometry has been collected. The authors attempt to describe how to plan a measurement, how to find the cell parameters, ho...
Samonaprawiające się lakiery
The recent decade brought about new dimensions to materials developments; stimuliresponse materials capable of responding to internal or external stimuli. The ability of materials to autonomously self-heal is the most pr...
Reakcja Niementowskiego
The Niementowski reaction depends on the fusion of anthranilic acid with carbonyl compounds or amides and dates back to the end of the nineteenth century. Numerous variations of the reaction components and modifications...