Microstereolithography-fabricated microneedles for fluid sampling of histamine-contaminated tuna
Journal Title: International Journal of Bioprinting - Year 2016, Vol 2, Issue 1
Abstract
A custom-designed microneedle sampling system was prepared using dynamic mask microstereolithography; this sampling system was used for determination of histamine content in fresh, histamine-spiked, and spoiled tuna flesh. Lateral flow (test strip) assays were successfully utilized in the microneedle sampling system to assess histamine content. Good agreement was noted between data obtained from the microneedle sampling system and a commercially available histamine detection kit. A discrepancy was noted in the results from the microneedle sampling system and the commercially available histamine detection kit at low (negative) levels of histamine. There was an improvement in the agreement between the microneedle sampling system and the commercially available histamine detection kit at higher histamine levels. The results, which showed an improvement in the test duration and the amount of reagent needed for histamine detection, indicate the promise of printed microneedle sampling systems for histamine detection in seafood samples and other types of food testing.
Authors and Affiliations
Ryan D. Boehm, Panupong Jaipan, Kai-Hung Yang, Thomas N. Stewart3 and Roger J. Narayan
Keywords
Related Articles
- EP ID EP678644
- DOI -
- Views 177
- Downloads 0
Bioprinting with human stem cell-laden alginate-gelatin bioink and bioactive glass for tissue engineering
Three-dimensional (3D) bioprinting technologies have shown great potential in the fabrication of 3D models for different human tissues. Stem cells are an attractive cell source in tissue engineering as they can be direct...
A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
3D bioprinting is an emerging technology that enables the fabrication of three-dimensional organised cellular constructs. One of the major challenges in 3D bioprinting is to develop a material to meet the harsh requireme...
Additive manufacturing of bone scaffolds
Additive manufacturing (AM) can obtain not only customized external shape but also porous internal structure for scaffolds, both of which are of great importance for repairing large segmental bone defects. The scaffold f...
Electrohydrodynamic printing process monitoring by microscopic image identification
Electrohydrodynamic printing (EHDP) is able to precisely manipulate the position, size, and morphology of micro-/nano-fibers and fabricate high-resolution scaffolds using viscous biopolymer solutions. However, less atten...
3D bioprinting for tissue engineering: Stem cells in hydrogels
Surgical limitations require alternative methods of repairing and replacing diseased and damaged tissue. Regenerative medicine is a growing area of research with engineered tissues already being used successfully in pati...