AN OVERVIEW ON HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS
Journal Title: Int J of Pharm Res & Analy - Year 2016, Vol 6, Issue 1
Abstract
ABSTRACT High performance Capillary electrophoresis (HPCE) is a separation technique in which the analytes are separated on the basis of differences in their charge-to-size ratios. This makes HPCE especially suitable for the analysis of molecules with abroad range of sizes, charge and hydrophobicity, as proteins and peptides. Another attractive feature of HPCE is its ability to handle minute sample amounts, with nano-litre injection volumes, which makes it ideal for applications when the sample volumes are limited, as often is the case in analysis of body fluids, single cells and other small volume analysis of biofluids. Electrophoresisisperformed innarrow-bore (25- to 75-J.m id), fused silica capillaries. High voltages (10 to 30 kV) and high electricfields (100 to 500 V/cm) are applied across the capillary. In addition, the small injection volume has often been considered as a drawback of the HPCE technique in analysis of complex biological samples, as well as the nature of its injection has represented a reproducibility problem in quantitative chemical analysis. Keywords: High performance, Capillary electrophoresis, Hydrophobicity, Injection, Electric field.
Authors and Affiliations
Sudheer Kumar N*, Shilpa KT, Ajitha A, Uma Maheshwara Rao V
Keywords
Related Articles
- EP ID EP90741
- DOI -
- Views 173
- Downloads 0
ATTENUATED TOTAL REFLECTANCE SPECTROSCOPY: AN OVERVIEW
Attenuated Total Reflectance (ATR) is today the most widely used FTIR sampling tool. ATR generally allows qualitative or quantitative analysis of samples with little or no sample preparation which greatly speeds sample a...
ANALYTICAL TECHNIQUES FOR THE ESTIMATION OF FENOFIBRATE IN BULK AND PHARMACEUTICAL DOSAGE FORMS: A REVIEW
Fenofibrate which is chemically propan-2-yl 2-{4-[(4-chlorophenyl) carbonyl] phenoxy}-methyl propanoate. It is mainly used to reduce cholesterol levels in patients at risk of cardiovascular disease. Like other fibrates,...
VALIDATION OF STABILITY INDICATING HPLC METHOD FOR ASSAY OF FUSIDIC ACID, BETAMETHASONE-17 VALERATE AND CHLOROCRESOL CONTENT IN TOPICAL PHARMACEUTICAL FORMULATION
A new stability indicating reversed phase HPLC method was developed and validated for assay of fusidic acid, betamethasone -17 valerate and Chlorocresol in Topical pharmaceutical formulation. The chromatographic separati...
A REVIEW ON TIME CORRELATED SINGLE PHOTON COUNTING
The phenomenon of molecular fluorescence emission contains both spectral and time information it is former which has traditionally been more usually associated with routine analytical applications of fluorescence spectr...
A NEW METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF ELETRIPTAN BY USING RP-HPLC METHOD
A simple, rapid, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for estimation of eletriptan in tablet dosage form. Chromatographic separation was carried out by...