CHARACTERIZATION OF L-ASPARAGINASE PRODUCING ENDOPHYTIC FUNGI ISOLATED FROM RIPENED FRUIT OF CAPSICUM FRUTESCENCE
Journal Title: International Journal of Pharmaceutical Development & Technology - Year 2014, Vol 4, Issue 1
Abstract
The main objective is to evaluate L-asparaginase production of endophytic fungi isolated from ripened fruit of Capsicum frutescence var US 341. In the present work endophytic fungal strains were screened for production of extra-cellular L-asparaginase, an anti-neoplastic agent used in the chemotherapy of lymphoblastic leukemia. L-Asparaginase activity of endophytic fungi was detected using modified Czapex dok’s medium containing L-asparagine and Phenol red as indicator. In the present study out of 16 fungal isolates, 6 fungal isolates were identified as L-asparaginase positive of which two fungal isolates RFEF-1 and RFEF-2 are identified as Aspergillus sp on their morphological characterization of mycelia, conidiospores and conidia under microscopic observation. The effect of growth parameters on biomass and production of L-asparaginase of these isolates have been characterized. Both isolates showed maximum biomass RFEF-1(2.42gms), RFEF-2(3.03gms) and L-asparaginase production RFEF-1(39.7 IU), RFEF-2(37.86 IU) at 35oC and pH -7.0.
Authors and Affiliations
Amrutha V. Audipudi , G. Naga Ratna Supriya , R. Pallavi , P. Ganga Mani
Keywords
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