Cloning and identification of the lobophorin biosynthetic gene cluster from marine Streptomyces olivaceus strain FXJ7.023
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2016, Vol 29, Issue 1
Abstract
A full length about 105 kb gene cluster containing 35 open reading frames involved in the biosynthesis of lobophorins was cloned and sequenced from a fosmid genomic library of Streptomyces olivaceus strain FXJ7.023. The cluster was identified by genome wide annotation and analysis of secondary metabolite biosynthesis gene clusters by anti SMASH and knockout of loading module-contained region of polyketide skeleton synthesis gene (the starter of lobS1). Gene cluster comparative analysis suggested that the cluster encoded the complete genes for lobophorin polyketide assembly, modification, substrate catalysis, regulation, transportation and resistance, and shows great identity to the newest reported lobophorin biosynthetic gene cluster from Streptomyces sp. SCSIO 01127, but with a significant gene rearrangement in the PKS modules.
Authors and Affiliations
Changwu Yue , Jing Niu , Ning Liu , Yuhong Lü , Minghao Liu , Yuanyuan Li , Miao Wang , Meiyun Shao , Shenyan Qian , Yuxin Bao , Ying Huang
Keywords
Related Articles
- EP ID EP128297
- DOI -
- Views 95
- Downloads 0
Standardization of micropropgation techniques for Aloe vera: A pharmaceutically important plant
Aloe vera (Syn Aloe barbadensis Mill.), a medicinal plant, has a great potential in cosmetic and drug industry due to presence of more than 200 bioactive compounds. Natural propagation of Aloe vera, by means of suckers,...
Haplophytin B from Maclurodendron porteri
An alkaloid from Maclurodendron porteri has been isolated and characterized. Extraction process was conducted by acid-base extraction method followed by column chromatography. The structure was established by nuclear mag...
The combination of zinc and glibenclamide limits cardiovascular complications in diabetic rats via multiple mechanisms
Cardiovascular complications have become a major cause of mortality for diabetic patients. Glibenclamide is an effective hypoglycemic agent, but failed to alleviate diabetic complications. This study aimed to evalu...
Analgesic, Anti-inflammatory and CNS depressant effects of Atropa belladonna
The present study was carried out to investigate, in vivo, analgesic, anti-inflammatory and neuro-pharmacological activities of the methanolic extract of Atropa belladonna. The analgesic activity was measured by ac...
Curative effect of amlodipine combined with enalapril in curing hypertension of the aged
This paper aims to research the clinical effect and security of using amlodipine and enalapril together to cure hypertension of aged people. Random number table was used to divide clinical data of 114 aged hyperten...