Development and validation of GC-MS method for analysis of chloropyramine hydrochloride in ointments
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2015, Vol 5, Issue 5
Abstract
A simple, rapid, sensitive, specific, accurate, and reproducible method for the determination of chloropyramine hydrochloride in ointments based on gas chromatography – mass spectrometry detection was developed and validated. Dissolution of chloropyramine hydrochloride in the ointment was performed with 0.5 M HCl. After alkalization with 25% (V/V) NH4OH, chloropyramine base was extracted with chloroform. The method was validated in respect of linearity, specificity, precision, recovery, limit of detection (LOD), limit of quantification (LOQ) and stability. In terms of performances: Recovery 90.0 – 98.7%, LOD 0.04 mg/g, LOQ 0.132 mg/g, specificity, selectivity and precision the proposed method was found suitable for routine analysis.
Authors and Affiliations
Vesna Kostik1* , Biljana Gjorgeska2 , Sofija Petkovska2
Keywords
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- EP ID EP132524
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