HPLC METHOD DEVELOPMENT AND VALIDATION: AN OVERVIEW
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 4
Abstract
ABSTRACT High performance liquid chromatography (HPLC) is an analytical technique which is proficient to separate, detect and quantify various drugs and its related degradants. An appropriate mobile phase, column, column temperature, wavelength and gradient must be found that affords suitable compatibility and stability of drug as well as degradants and impurities. This review covers the importance of RP-HPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters need to be optimized for an efficient method development. Keywords: Method validation, Method development, High PressureLiquid Chromatography (HPLC).
Authors and Affiliations
G. Santhosh*, G. Nagasowjanya, A. Ajitha, Y. Uma Maheswara Rao
Keywords
Related Articles
- EP ID EP143893
- DOI -
- Views 115
- Downloads 0
AN OVERVIEW OF ELECTRON PROBE MICROANALYSIS
ABSTRACT Electron Probe Microanalysis (EPMA) is an analytical technique that is used to establish the composition of small areas on specimens. It’s one of the several particle-beam techniques These analytical te...
DIFFERENCE ABSORBANCE SPECTROPHOTOMETRIC DETERMINATION OF OFLOXACIN AND ORNIDAZOLE IN DOSAGE FORM
ABSTRACT Describing the simple, precise and rapid method based on difference absorbance spectroscopic technique for the determination of Ofloxacin (OF) and ornidazole (OZ) in tablet dosage form. The projected met...
ANALYTICAL PROCESS OF DRUGS BY ULTRAVIOLET (UV) SPECTROSCOPY – A REVIEW
ABSTRACT The pharmaceutical analysis comprises the procedures necessary to determine the “identity, strength, quality and purity” of such compounds. It also includes the analysis of raw material and intermediates...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF MULTICOMPONENTS IN BULK AND TABLET DOSAGE FORM
ABSTRACT A simple, accurate, precise, and sensitive and a highly selective UV-spectrophotometric method were developed for the simultaneous estimation of atorvastatin calcium and pyridoxine hydrochloride at a ma...
METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC ANALYSIS OF BROMFENAC IN RABBIT PLASMA
ABSTRACT A simple, precise, rapid, and accurate reverse phase high performance liquid chromatography method was developed for the estimation of Bromfenac in Rabbit plasma by using Paracetamol as IS. The protein p...