Mutagenic breeding of Quambalaria cyanescens strain for the production of milk-clotting enzymes
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 3
Abstract
A strain for producing rennet was screened from red kojic rice and identified as Quambalaria cyanescens. In the current work, various mutagenic treatments, such as UV, diethyl sulfate and their combinations were applied for improving the production of milk-clotting enzymes. The mutagenic conditions were optimized based on the fatality rate of the strain. A significant increasing in the productivity of the fungal rennet produced from the strain Quambalaria cyanescens was achieved and its milk-clotting activity (MCA) was increased from 57 to 117 (SU mL-1) through the mutagenic breeding. Further study showed that MCA was greatly inhibited (P<0.0001) by pepstain A, indicating it belongs to an aspartic acid protease, but absence of serine protease, metalloproteinase and cysteine protease. The mutated strain with the highest activity of milk-clotting enzymes showed a stable capacity to produce rennet through the test of its heritability. This is the first report of the breeding study of Quambalaria cyanescens for its capacity to produce microbial rennet.
Authors and Affiliations
Chengzhong Wang , Hongyan Rao , Zhongkai Zhou , Yan Zhang
Keywords
Related Articles
- EP ID EP141920
- DOI -
- Views 64
- Downloads 0
Clinical curative effect of Percutaneous vertebroplasty combined with 125I-seed implantation in treating spinal metastatic tumor
This paper selected and studied 15 in-hospital patients to analyze and discuss the clinical curative effect of percutaneous vertebroplasty (PVP) combined with 125I-seed implantation in treating spinal metastatic tu...
Molecular characteristics of extractives of Hylocereus undulates stems
As one of traditional and dominant species of famous fruits in South China, Hylocereus undulates is considered as the important fruit resources, however, the constituent properties of Hylocereus undulates stems hav...
The market of probiotics
The advertising of probiotics in diary products on the market has claimed several health-improving properties, including prevention and treatment of obesity, cardiovascular diseases and cancer prophylaxis, osteopor...
Q192R paraoxonase1 polymorphism is a risk factor for cataract in Pakistani population
Cataract, the lens opacity, is among major causes of blindness in Pakistani population. In recent past, oxidative stress is suggested to play crucial role in loss of transparency. Along with other antioxidants, Par...
Anti-HIV-1 activities of extracts and phenolics from Smilax china L.
Four extracts (EtOH, CHCl3, EtOAc, and BuOH) and five phenolics (dihydrokaempferol (1), resveratrol (2), kaempferol-7-O-β-D-glucoside (3), dihydrokaempferol-3-O-α-L-rhamnoside (4), oxyresveratrol (5)) from Smilax c...