Quality evaluation of Houttuynia cordata Thunb. by high performance liquid chromatography with photodiode-array detection (HPLC-DAD)
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 2
Abstract
Abstract: A new, validated method, developed for the simultaneous determination of 16 phenolics (chlorogenic acid, scopoletin, vitexin, rutin, afzelin, isoquercitrin, narirutin, kaempferitrin, quercitrin, quercetin, kaempferol, chrysosplenol D, vitexicarpin, 5-hydroxy-3,3',4',7-tetramethoxy flavonoids, 5-hydroxy-3,4',6,7-tetramethoxy flavonoids and kaempferol-3,7,4'-trimethyl ether) in Houttuynia cordata Thunb. was successfully applied to 35 batches of samples collected from different regions or at different times and their total antioxidant activities (TAAs) were investigated. The aim was to develop a quality control method to simultaneously determine the major active components in H. cordata. The HPLC-DAD method was performed using a reverse-phase C18 column with a gradient elution system (acetonitrile-methanol-water) and simultaneous detection at 345 nm. Linear behaviors of method for all the analytes were observed with linear regression relationship (r2>0.999) at the concentration ranges investigated. The recoveries of the 16 phenolics ranged from 98.93% to 101.26%. The samples analyzed were differentiated and classified based on the contents of the 16 characteristic compounds and the TAA using hierarchical clustering analysis (HCA) and principal component analysis (PCA). The results analyzed showed that similar chemical profiles and TAAs were divided into the same group. There was some evidence that active compounds, although they varied significantly, may possess uniform anti-oxidant activities and have potentially synergistic effects.
Authors and Affiliations
Zhan-nan Yang , Yi-ming Sun , Jin-wu Chen , Shi-qiong Luo , Zheng-wen Yu , Min Sun
Keywords
Related Articles
- EP ID EP120997
- DOI -
- Views 112
- Downloads 0
Antiproliferative xanthone derivatives from Calophyllum inophyllum and Calophyllum soulattri
Structure-activity relationships of eleven xanthones were comparatively predicted for four cancer cell lines after the compounds were subjected to antiproliferative assay against B-lymphocyte cells (Raji), colon ca...
Apoptotic and antioxidant activities of methanol extract of Mussaenda roxburghii leaves
Present research work was designed to study the anticancer and antioxidant activities of methanol extract of Mussaenda roxburghii. Anticancer activity of MMR has been carried out on Ehrlich ascites carcinoma (EAC)...
Serotonergic activity and hypothalamic-pituitary-adrenal axis response in alcohol administered and subsequently withdrawn rats
Present study aims to depict the role of serotonergic pathways in discrete brain areas (hypothalamus, amygdala, and hippocampus) and their interaction with hypothalamic pituitary adrenal (HPA) axis in alcohol depen...
Exploiting the interaction of polymethacrylates with iron oxide for the enhancement of mucoadhesive strength
Abstract: The present investigation was aimed at preparation of polymethacrylate(s)- iron oxide conjugates and to evaluate mucoadhesive performance using texture analyzer. Eudragit RL-iron oxide or Eudragit RS-ir...
Comparative bioavailability and pharmacokinetic study of Cefadroxil capsules in male healthy volunteers of Pakistan
The current study was aimed to judge bioequivalence between two formulations of cefadroxil capsules as guided by FDA guidelines. Another objective was to conduct pharmacokinetic evaluation in Pakistani population....