The Development Extract Protein of Bambara Nut (Vigna subterranea (L.) Verdc.) As a Reagent for Detecting Food Allergies on Skin Prick Test Method
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2015, Vol 5, Issue 3
Abstract
This study aimed to prepare the protein extract of bambara nut seeds, characterize and apply as a reagent for Skin Prick Test (SPT). Bambara nut protein was extracted from seeds flour of Bambara nut by isoelectric precipitation then analyzed by using SDS-PAGE. The protein extract was formulated into a SPT reagent and met the requirements of the European Pharmacopoeia Monograph on Allergen Products 7 (2010:1063). The SPT reagent was applied on 11 adult subjects who have been suffered food allergies and 9 adult individuals non food allergies. Sera of subjects were collected to measure the IgE total and specific IgEbinding. Immunobloting were also performed on sera of bambara nut allergies. The number of bands were detected in protein extract of bambara nut were 14 bands with the molecular weight of 17-122 kDa. The SPT results suggest that diameter of wheal were ≥ 5 mm. Sensitivity of extract bambara nut protein was 91% and a negative error of 9%, whereas specificity of those was 100% and an error rate of positive diagnosis occurrenceat 0%. Two sera subjects of these bambara nut allergic participants showed the specific binding to allergen of bambara nut
Authors and Affiliations
Sri Yadial Chalid1 , Dahrul Syah2 , Puspo Edi Giriwono2 Fransiska Rungkat Zakaria2,
Keywords
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