Neuroprotective effect of Baillonella toxisperma Pierre on the oxidative stress status in an experimental animal model of Alzheimers disease
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2014, Vol 5, Issue 3
Abstract
Recent research findings have shown that neurodegenerative disorders like Alzheimer are partly caused by increased levels of reactive oxygen species. The present study aims to investigate the effect of Baillonella toxisperma on an oxidative stress status induced by aluminium chloride in female rats.  Hydroethanolic extract of B. toxisperma (HEEBT) was selected after evaluation of antioxidant potential using four methods notably TAC (total antioxidant capacity), scavenging of 1, 1-Diphenyl-2-Picrilhydrazyl (DPPH), hydroxyl radical (·OH) and inhibition of lipid peroxidation. It was used to evaluate neuroprotection in female albino wistar rats that were intoxicated with aluminium chloride. Twenty-five rats were divided into five groups of five rats each; group 1: control group, group 2: AlCl3 exclusively treated (32.5 mg/kgBW) and served as positive control; group 3, 4 and 5 were treated with AlCl3 and either 150/300mg/kgBW of HEEBT or 100mg/kgBW vitamin E daily for three days 24H after administration of AlCl3. After treatment, animals’ blood and brain were collected. Hydroperoxide (ROOH), Malondialdehyde (MDA) and protein thiol levels were determined in brain homogenates while ALT, AST, protein and creatinine levels were evaluated in plasma and catalase in erythrocytes. The in vitro antioxidant activity of the HEEBT was better than for ethanolic and aqueous extracts. Results of biochemical analysis of brains of female rats revealed that AlCl3 significantly (p<0,05) increased MDA levels (3,97 vs 7.32 µM) and the activity of catalase (0.020 vs 0.096 mMH2O2/min/mg of proteins) in female rats. In contrast, female rats treated with HEE of B. toxisperma (150 and 300 mg/kg/day b.wt) showed a significant decrease (p<0,05) of MDA levels (4.43 and 4.58 µM) and the activity of catalase (0.045 and 0.054 mMH2O2/min/mg of proteins). The results obtained for toxicity markers (AST, ALT and creatinine) have the same profile. Hydroethanolic extract of B. toxisperma have antioxidant and neuroprotective effects by scavenging free radical and protecting neurons cells against oxidative damage induced by aluminum chloride.
Authors and Affiliations
Djiokeng Paka Ghislain, Ngoumen Ngassa Dany Joel, Youovop Fotso Janvier, Mbong Angie Mary-Ann, Ngondi Judith Laure, Julius Enyong Oben
Keywords
Related Articles
- EP ID EP654417
- DOI 10.24297/jab.v5i3.5558
- Views 174
- Downloads 0
Assessment of the insecticidal activity of aqueous extracts of two local plants for the management of Leucinodes orbonalis Guenee, (Lepidoptera : Pyralidae ), pest of eggplant (Solanum aethiopicum), in guinean area of Cote d' lvoriee
Leucinodes orbonalis is a major pest of eggplant (Solanum aethiopicum). Control tests with aqueous extracts of two local plants have been carried out on this pest in Azagui located Guinean area of Co´te d'Ivoire. The exp...
Comparative study of some parameters biological of Bactrocera dorsalis and Ceratitis cosyra (Diptera :Tephritidae) pests of mango (Mangifera indica) in Co´te d Ivoire
Bactrocera dorsalis and Ceratitis cosyra are the major pests of mango (Mangifera indica), in Cote d'Ivoire. The study of biological parameters of these pests has revealed that the development of B. dorsalis is higher tha...
Impact of herbicides Imazethpayr (Pursuit) and Oxyfluorfen (Goal) on weed control and yield of groundnut (Arachis hypogaea L.)
Trials were initiated in 2007 to evaluate systems of weed management in peanut using herbicides. Two trials were conducted at Sudan University of Science and Technology, College of Agricultural Studies and Research Farm...
The effect of jujube oil on some biological activities of Culex pipiens mosquito
Synthetic insecticides are quick, effective and most popular methods of pest control. But because of their unfavorable effects on environment and non-target organisms, there is a growing need to find new, safe effective...
Isolation and Characterization of Polyaromatic Hydrocarbons Degrading Bacteria from Compost Leachate
Polycyclic aromatic hydrocarbon (PAHs) degrading bacteria were isolated from compost leachate (CL) that collected from a composting site located in central Scotland, UK. Isolation was carried out by enrichment using phen...