Novel Herbal Storage Media for Exarticulated Teeth
Journal Title: International Journal of Contemporary Medical Research - Year 2016, Vol 3, Issue 3
Abstract
Introduction: Exarticulation is one of the most severe forms of dental trauma. Ideally, the tooth should be replanted immediately after the injury for better prognosis. Unfortunately, immediate repositioning of the tooth is not always possible. Recently researchers have proven that polyphenols can help in maintaining PDL cell viability. Such polyphenols are found in many herbal products like Cameillia sinensis, Punicia granatum, Vaccinia macrocarpon, Prunus domestica and Psidium guajava leaves which are not yet explored as storage media except Camellia sinensis. These herbal products being readily available worldwide might prove as a boon for effective storage capacity and maintenance of cell viability. This study was aimed to assess and compare the viability of periodontal ligament fibroblasts in the above mentioned herbal media. Materials and methods: A strain of periodontal ligament fibroblasts was established from healthy premolar tooth extracted for orthodontic purpose and cultured in Dulbeco’s Modified Eagle’s medium. The cultivated cells were exposed to the different study media at 15mins, 30 mins, 1 hour and 3 hours. Cell viability was assessed Neutral red assay. The results obtained were statistically analysed. Results: Vaccinium macrocarpon and Punicia granatum had greater mean optical density compared to the other study media. The optical density decreased as the time intervals increased. The viability reduction at 15 mins- 3 hours time intervals was seen least with Vaccinium macrocarpon which was 24.7%. (p<0.05). Conclusion: Vaccinium macrocarpon can be used as potential storage media. Punicia granatum, Prunus domestica, Psidium guajava leaves and Camellia sinensis showed good cell viability.
Authors and Affiliations
Rajesh T. Anegundi, Shukla Nisha Paritosh, Anand Tavargeri, Shruthi Patil, Vijay Trasad, Prashant Battepati
Keywords
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