Occult HBV among anti-HBc + alone and not alone samples: mutation analysis of S Gene
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 7
Abstract
Objective To investigate the status of occult hepatitis B virus infection(OBI) among blood donors in Quzhou, Zhejiang, and to analyze the mutation of S region in blood donors with anti-HBc+ alone and non anti-HBc+ alone. Methods The OBI samples were screened by ELISA and NAT; the HBV DNA was amplified and sequenced; 20 anti-HBc+ alone and 25 not anti-HBc+ alone samples were obtained. Results The detection rate of OBI in Quzhou was 0.10%(155/161 045), and the positive rate of anti-HBc was 74.19%(115/155). The detection rate of OBI increased with the age of blood donors(P<0.05), but was not related to gender. The positive rate of anti-HBc+ alone was 22.58%(35/155), and that of not anti-HBc alone was 51.61%(80/155). Among the 45 OBI sequencing samples, the proportion of B and C genotype was 73.33%(33/45) and 20.00%(9/45), respectively. The mutation sites of blood donors in the anti-HBc+ alone group were more than those in the not anti-HBc+ alone group, and the mutation rates of S114T and V168A on MHR were significantly different(P<0.05). Conclusion The genotype of OBI infection in Quzhou is mainly type B. The mutation sites of blood donors with anti-HBc+ alone are higher than those with not anti-HBc+ alone, which may be more suitable as one of the OBI screening indicators.
Authors and Affiliations
Jianxun ZHENG, Shiquan WU, Zhenzhen LIU, Sujun JIANG
Keywords
Related Articles
- EP ID EP742816
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.07.013
- Views 43
- Downloads 0
Performance verification of domestic nucleic acid testing system in blood stations based on T/CSBT
Objective To explore the performance verification of the Shengxiang automatic NAT system for HBV DNA, HCV RNA and HIV RNA-1 using PCR-fluorescence in the laboratories of blood stations, in order to meet the requirements...
Establishment of an resilience evaluation index framework for blood service system under emergency support
Objective To construct an index framework for the evaluation of blood service system resilience under emergency support, and improve the ability of blood centers to deal with emergencies. Methods The contents of evaluati...
Suspected hemolysis caused by type A therapeutic plasma exchange in a patient with AB subtype miss detected
Objective To investigate the serological changes of blood type and whether hemolytic reactions occurred in a patient with ABw subtype after type A therapeutic plasma exchange(TPE) retrospectively. Methods The ABO blood g...
Preparation of a novel double polymerized of red blood cell substitute and preliminary observation of its curative effect
Objective To study the red blood cell substitute preparation method of glutaraldehyde(GDA) and Bis(3, 5-dibromosalicyl) fumarate(DBBF) double polymerized, and also observe its curative effect. Methods The affecting facto...
Analysis of laboratory tests and prevention strategies for hemolytic disease of the fetus and newborn caused by anti-M
Objective To analyze the application of serological test results in the diagnosis and treatment of anti-M-induced hemolytic disease of the fetus and newborn(HDFN), and to explore HDFN prevention strategies. Methods The s...