Performance of Antibiogram on Slide in the Diagnosis of Multiresistant Tuberculosis Persons Living with HIV / AIDS in Kinshasa
Journal Title: International Journal of Medical Research Professionals - Year 2017, Vol 3, Issue 5
Abstract
Background: Multidrug-resistant tuberculosis (MDR-TB) is a real public health problem in the world. Its rapid and early diagnosis remains an important means of reducing its morbidity and mortality. Ziehl-Neelsen staining, which is common in tuberculosis detection and treatment centers, does not lead to the diagnosis of drug-resistant TB. Objective: The objective of the study was to evaluate the performance of the slide antibiogram in the detection of resistance to rifampicin and to second-line molecules. Methods: Prospective analysis of sputum smear from tuberculosis patients in therapeutic failure or relapse situations, comparing the performance of the antibiogram to slide technique against the gold standard, the method of proportion on medium Of LJ and the molecular method of Gen-Xpert® MTB / RIF in the detection of resistance to rifampicin. Results: Resistance to rifampicin was observed in 66.3% of samples (53) using the slide antibiogram test, 67.5% (54) with Gen-Xpert® MTB / RIF and 61.3% (49) by the proportional method. The MDR-TB rate was 56.3%, 15% pre XDR-TB and 1.3% XDR-TB. The sensitivity and specificity of the antibiogram on slide was 100% and 87.1% with a positive predictive value of 92.5%. Gen-Xpert® MTB / RIF, however, was 100% and 83.9%, and the positive predictive value was 90.7%. Conclusion: Slide antibiogram showed its performance against LJ and Gen-xpert in detecting resistance to rifampicin and / or second-line molecules (Ofloxacin and Kanamycin) after 10 days of incubation. Its popularization deserves consideration in resource-limited countries.
Authors and Affiliations
Kabedi Bajani MJ, Kayembe Ntumba JM, Kayembe Kalambayi P, Kashongwe Munogolo Z, Lunguya Metila O, Mujangi Kadima B, Bisuta Fueza S, Mbaya Kalumba P, Taba Kalulu M, Muyembe Tamfum JJ
Keywords
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- EP ID EP547585
- DOI 10.21276/ijmrp.2017.3.5.054
- Views 86
- Downloads 0
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