Phenotypic and Genotypic Identification of Acinetobacter baumannii with Special Reference to Blaoxa-51Like Gene and its Antimicrobial Susceptibility Pattern from Intensive Care Unites in Kanpur
Journal Title: International Journal of Contemporary Medical Research - Year 2017, Vol 4, Issue 5
Abstract
Introduction: Acinetobacter baumannii are important nosocomial pathogens being increasingly reported worldwide in critically ill patients. Resistance to multiple antimicrobial agent are major concern for all clinical settings. So this study was conducted to identify the Acinetobacter baumannii from different clinical samples in Kanpur. Material and Methods: A total of 200 clinical cases were observed for Acinetobacter baumannii isolation by the standard phenotypic microbiological procedures. Antimicrobial resistant pattern was analysed by as per CLSI guidelines 2016. And intrinsic gene blaOXA-51 like were also detected by PCR. Results: In this prospective study 34 Acinetobacter baumannii were isolated from 200 cases but 10% were found to be infected. The higher percentage of isolates were from respiratory samples 24 (70.58%) followed by central line 5 (14.70%), blood 2 (5.88%), urine 1 (2.9%) and foley’s tip 1 (2.9%). Isolates were highly resistant to most of the antimicrobial agents. Antibiotic susceptibility analyses of the isolates revealed that the resistance to penicillin and cephalosporins were most common followed by 85.29% resistance to gentamicin, and 79.41% resistance to imipenem. All isolated acinetobacter were susceptible to tigecycline and polymyxin B. Conclusion: A high level of antibiotic resistance was observed in our study. Carbapenamase producing gene blaOXA-51like gene intrinsically present in A.baumanni. Therefore presence of blaOXA-51 like gene was found to be confirmation for A.baumannii species. Thus judicial use of antimicrobials & adopting strict infection control policies will be useful.
Authors and Affiliations
Nidhi Pal, R Sujatha, Anil Kumar
Keywords
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