Plant Regeneration from Nodal explants Culture through Organogenesis in Eupatorium triplinerve vahl
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 4
Abstract
An efficient plant regeneration protocol was developed for Eupatorium triplinerve Vahl, an important medicinal herb. Nodal explants inoculated on Murashige and Skoog medium supplemented with 4.44 μM 6-Benzyladenine(BA), showed better growth response and produced 18.3±1.15 number of shoots per explant with an average length of 6.4± 0.06 cm after 30 days. Rooting of shoots was achieved in half strength MS medium supplemented with 2.46 μM Indol-3-butyric acid, this produced 6.02 ± 0.2 number of roots per shoot with an average height of 3.5 ± 0.1 cm after 30 days. The rooted plantlets were transferred in the pots hardening, 80 percent of plantlets survived and resumed growth in the mixture of soil, vermiculite and farm yard manure (1 : 1 : 1).
Authors and Affiliations
*S. Usha1, Karpagam, S 2
Keywords
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- EP ID EP389986
- DOI 10.9790/3008-1204032832
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