QTLs Mapping of Morpho-Physiological Traits of Flag Leaf in Steptoe × Morex Doubled Haploid Lines of Barley in Normal and Salinity Stress Conditions
Journal Title: International Journal of Farming and Allied Sciences - Year 2016, Vol 5, Issue 5
Abstract
Salt tolerance is a complex trait that involves multiple physiological and biochemical mechanisms and numerous genes. The identification of genes whose expression enables plants to tolerance salt stress is essential for breeding programs. In order to a doubled haploid line (DHL) population with 72 lines derived from a cross of ‘Steptoe/Morex’ was used to identify quantitative trait loci (QTL) of morpho-physiological traits in barley (Hordeum vulgar L.). This experiment was carried out in the Research Farm, College of Agriculture, University of Zabol, Zabol, Iran, in growing year 2012. It was arranged as in two randomized complete block design (RCBD) with two replicates and two normal and salinity stress conditions. Morpho-physiological traits of DHLs were measured in two environments. Analysis of variance (ANOVA) results showed that there were significant differences among the lines. Transgressive segregation in both directions was significant for flag leaf length, flag leaf width, flag leaf area and chlorophyll contents. The strongest positive correlations were observed between flag leaf length and flag leaf width (r = 0.994**). QTL analysis was conducted by composite interval mapping (CIM) method separately for each trait in each conditions and mean of two conditions. Twenty-nine QTLs were identified for the studied traits. Phenotypic variance explained by these QTLs variable from 8.66 to 29.94. Highest LOD scores were obtained for flag leaf area (QFla2n) on 2H chromosome in normal conditions.
Authors and Affiliations
Habibeh Shahraki, Barat Ali Fakheri
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