Role of p16, Ki67 and CK17 in differentiating benign lesions, cervical intraepithelial neoplasia(CIN) and atypical immature squamous metaplasia(AIM) of uterine cervix
Journal Title: Pathology Update: Tropical Journal of Pathology and Microbiology - Year 2017, Vol 3, Issue 1
Abstract
Cervical intraepithelial neoplasia (CIN) is a premalignant lesion capable of progressing to cervical cancer. Despite the existing well-defined criteria, the histopathological diagnosis is subject to high rates of discordance among pathologists. Aim: To study the role of p16, Ki67 and CK17 in differentiating benign lesions, cervical intraepithelial lesions(CIN) and atypical immature squamous metaplasia (AIM)and to improve intra and interobserver reproducibility of diagnosis of cervical neoplasia. Material and Methods: In a cross sectional study, a total of 75 cervical biopsies including benign lesions (n=24), AIM (n=28), CIN (n=23) were studied and analyzed immunohistochemically using p16, Ki67 and CK17 immunomarkers. Data was evaluated using chi-square test. Results: p16 and Ki 67positivity were observed in 91.3% and 78.26% of CIN and 28.57% of AIM respectively. None of the benign lesions expressed p16 and Ki67while CK17 positivity was observed in 46.42% of CIN and 100% of AIM with 12.5% of benign lesions. Conclusion: The three biomarkers (p16, CK17 and Ki67) had a high degree of sensitivity and specificity and appear to be a useful and reliable diagnostic adjunct to improve the routine diagnosis and reduce interobserver variability in cervical biopsy specimens. Immunohistochemical markers such as p16 alone or with Ki67 represents important tool for the pathologists in distinguishing high grade cervical dysplasia from its benign mimics such as AIM and reactive inflammatory lesion thus avoiding overtreatment.
Authors and Affiliations
Shaila K Mitra, Rajiv K Misra, Bharti Varshney, Ankita Kumari, Divya Rai
Keywords
Related Articles
- EP ID EP262949
- DOI 10.17511/jopm.2017.i1.03
- Views 149
- Downloads 0
Clinico-pathological study of benign soft tissue tumors: a study from tertiary teaching hospital
Background: Aim of the study was to analyze the histopathological findings in various benign soft tissue tumors and to study its frequency distribution in relation to age, gender and anatomical site. Material and Method...
A study of neoplastic study of gastrointestinal tumors
Background: Gastrointestinal tumors account for a large proportion of all neoplasms. The tumors of the Gastrointestinal tract pose a major problem in oncology and also are a major cause of apprehension in patient with th...
Histopathological overview of cystoscopic bladder biopsies- A retrospective analysis
Background: Wide spectrums of nonneoplastic and neoplastic lesions are observed in urinary bladder. Correlation between clinical findings and histopathological features is essential in arriving at a correct diagnosis. Th...
Pap smear as early diagnostic tool for cervical cancer- A life saviour
Background: Cervical cancer is a leading cause of mortality and morbidity among women globally and most common gynaecological cancer in developing countries. Papanicolaou smear study is a simple and cost effective screen...
Histopathological profile of sinonasal lesions with brief clinical correlation: experience in a tertiary care centre
Background: Sinonasal lesions are a common finding in Otorhinolaryngology outpatientdepartment. Most commonly they present withnasal obstruction. Clinically many of these lesions resemble eachother but they have multiple...