Serodiagnosis of tuberculosis by indigenously developed antibody detection with Indirect ELISA
Journal Title: Medpulse International Journal of Biochemistry - Year 2018, Vol 8, Issue 1
Abstract
Background: India is the country with the highest burden of tuberculosis (TB). The World Health Organization (WHO) TB statistics for India for 2016 give an estimated incidence figure of 2.79 million cases of TB for India. We tried to work on rapid diagnosis of TB by developing an indirect ELISA for antibodies against secretory antigens of H37Ra strain which will be indigenous, cost-effective and have better results than market available tests. Materials and Methods: A total of 50 confirmed pulmonary tuberculosis Sputum positive patient were selected. Again 50 sera samples were collected. 50 diseases control sera samples from various diseases (viz., Pneumonia, Lung abscess, Chronic Bronchitis, Non-tuberculous pleural effusion) were taken. We M. tuberculosis H37Ra strain was procured from IMTEK Chandigarh, India and isolated Secretory TB Ag by culturing this bacteria in a protein-free Saton’s medium anti-TB IgG from the sera of TB infected patients. We utilized this TB antigen for the development of Indirect ELISA for tubercular Antibody detection and tested them in above mentioned subject samples. Results: The sensitivity to detect TB Ab in Pulmonary tuberculosis (PTB) cases was found to be 94%. Likewise, the specificity of the test was 88%. The positive predictive value and negative predictive value was 94% and 88.88% and efficacy of the test was 92.20%. Conclusion: We successfully developed and tested indigenously developed Indirect ELISA for tubercular antibody detection. Further work is required to increase stability and effectiveness of this kit and to test its efficacy in extra pulmonary tuberculosis.
Authors and Affiliations
Kamlesh Manohar Palandurkar, Kiran Rajendra Giri Rajendra Giri, Reena Rajendra Giri
Keywords
Related Articles
- EP ID EP442131
- DOI 10.26611/1002814
- Views 169
- Downloads 0
Study of analyte stability time of serum electrolytes in a tertiary care public hospital
Background: The collected and shipped blood samples are exposed to various extra-analytical factors prior to analysis. At times there is a delay in sample analysis after centrifugation due to a large sample load, breakdo...
Erythrocyte CAT, GPx and GR levels in pregnant anemic woman
Pregnancy is characterized by a high-energy demand and an increased oxygen requirement. This is a condition exhibiting increased susceptibility to oxidative stress. Hydrogen peroxide is a harmful by-product of many norma...
Serum lipid profile in diabetic retinopaty – A case control study
Background: Uncontrolled hyperglycemia has been recognized to be in association with high incidence of microvascular complications like retinopathy and nephropathy in Type II diabetes mellitus. Diabetic retinopathy (DR)...
A study of diagnostic utility of serum ischemia modified albumin and IMA index in ischemic stroke and hemorrhagic stroke patients
Background: Stroke is associated with high morbidity and mortality rates, however, biochemical markers to aid in its early detection are lacking. As Ischemia Modified Albumin (IMA) is a novel marker for ischemia and prev...
Serum adenosine deaminase levels in patients with type 2 diabetes mellitus and its relation with fasting blood glucose and glycated hemoglobin levels
Background: Insulin resistance and decreased insulin secretion are the main physiological abnormalities of Type 2 Diabetes Mellitus (DM). Cell mediated immune system disturbance and improper T lymphocyte function also co...