STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF TRAVOPOST AND TIMOLOL IN BULK AND PHARMACEUTICAL DOSAGE FORMS
Journal Title: Int J of Pharm Rev& Res - Year 2015, Vol 5, Issue 3
Abstract
A Simple, precise and stability indicating RP-HPLC method for simultaneous estimation of Travopost and Timolol in bulk and pharmaceutical dosage form has been developed and validated.The chromatographic separation was carried out on Intersil ODS (150X4.6mm, 5μ) column using acetonitrile: buffer in the ratio of 10:90 % v/v as mobile phase .The flow rate was 1ml/min and effluent was detected at 213nm.The retention time of Travopost and Timolol were found to be 2.516 and 6.198min respectively. The method was linear over the concentration range of 12.5-75 μg/ml and 10-60 μg/ml for Timolol and Travopost respectively. The method was found to be precise as indicated by the repeatability analysis showing %RSD less than 2.Percentage recoveries for Travopost and Timolol were 100.27 and 100.41% respectively .All the validation parameters were determined and found within the limits as per ICH guidelines, which indicates validity of the method. Travopost and Timolol subjected to the stress conditions of acid, base, peroxide, thermal, UV& neutral degradation. The degradation products were well resolved from the main peak and its impurities, proving stability indicating ability of the method
Authors and Affiliations
R. V. Valli Kumari , P. VenkateswarRao , A. Sunitha , K. Keerthy
Keywords
Related Articles
- EP ID EP106565
- DOI -
- Views 161
- Downloads 0
ANTIOXIDANT AND ANTICOAGULANT ACTIVITY OF NOVEL NSUBSTITUTED 4 METHYL 5,7 DI HYDROXYL COUMARIN AND ITS ESTER DERIVATIVES
Some novel N- Substituted 4 methyl 5,7 di hydroxyl coumarins were synthesized efficiently in two methods general synthesis and microwave method in 4 steps. Both the methods 12 compounds were synthesized. The synthesized...
LIQUISOLID SYSTEMS - AN EMERGING STRATEGY FOR SOLUBILIZATION & DISSOLUTION RATE ENHANCEMENT OF BCS CLASS-II DRUGS
Now-a day‟s efforts are ongoing to enhance the oral bioavailability of lipophilic drugs/water insoluble drugs in order to increase their clinical efficacy. One of the most popular approaches includes Liquisolid technique...
CHRONOMODULATED DRUG DELIVERY SYSTEM: A REVIEW
In this review, the concepts of biological rhythms, chronobiology, chronopharmacology, and chronotherapy for various diseases have been discussed. Chronopharmaceutical Drug Delivery Systems (ChrDDS) is novel system which...
IN VITRO ANTIOXIDANT ACTIVITY OF VARIOUS EXTRACTS/FRACTIONS OF IXORA PAVETTA
Free radicals play an important role in the development of tissue damage and pathological events in living organism. There is a compelling evidence indicates that increased consumption of antioxidants from fruits and veg...
ANALYSIS OF OIL COMPOSITION OF THE CORIANDRUM SATIVUM LINN FRUIT BY SOXHLATION AND MACERATION TECHNIQUE
Coriandrum sativum Linn [Family Apiceae] is a medicinal herb used in the treatment of various disease and disorders. The present investigation was carried out to determine the change in the oil composition of the C.sativ...