STAINING OF HISTOLOGICAL SECTIONS FROM THE SMALL INTESTINE USING HIBISCUS SABDARIFFA
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 5
Abstract
Objective: this was a descriptive study aimed to assess the quality of staining of intestinal tissue by Hibiscus Sabdariffa solution compared to Hematoxylin-Eosin routine stain. Methods: paraffin-embedded formalin-fixed tissue sections from small intestine were stained by Hibiscus Sabdariffa solution using different concentrations and time durations in room temperature. Results: With 5 % solution, 48 % of slides showed at least very good quality of staining. The excellent results were mainly obtained when time duration was 60 minutes while poorest results were obtained when staining time was 1-2 minutes Conclusion: Hibiscus Sabdariffa solution is a cheap, natural, and harmless dye that can be used for staining of histological sections and it is comparable to the routine Hematoxylin and Eosin stain.
Authors and Affiliations
Abd-Alhafeez Ibnouf*| Department of Medical Laboratories, Portsudan Ahlia College, Portsudan, Sudan., Khalid Adam| Department of Molecular Biology, Faculty of Laboratory Medical Science, Neelain University, Khartoum, Sudan., Esam AbdulRaheem| Department of Medical Laboratories, College of Applied Medical Science, Shaqra University, Saudi Arabia., Ali Ageep| Department of Pathology, Red Sea University, Portsudan, Sudan.
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