Storage of the Recombinant Protein hPDGF-BB in the Culture of Pichia pastoris
Journal Title: SSR Institute of International Journal of Life Sciences (SSR-IIJLS) - Year 2018, Vol 4, Issue 4
Abstract
Human platelet-derived growth factor-BB (hPDGF-BB), a proliferation factor, has been successfully manufactured and approved by FDA as the treatment for diabetic foot ulcer and self bone grafting. There have been no reports on the storage of the recombinant hPDGF-BB (rhPDGF-BB) in the Pichia pastoris fermentation broth although during the research of process development and the production manufacture it needs to be stored at low temperature. The concentration of rhPDGF-BB protein in the fed-batch fermentation broth of P. pastoris was stable during 3-week storage at -20oC, but its bioactivity was reduced by 20%. The addition of a mixture of 50% glycerol with either 1 mM EDTA or 1 mM PMSF into the fermentation broth could fully preserve the bioactivity of rhPDGF-BB until 3 weeks at -20oC. The addition of 50% glycerol with either 1 mM EDTA or 1 mM PMSF was found no affection on the protein purification process.
Authors and Affiliations
Vu Minh Pham, Huy Hua Hoang Quoc, Huong-Xuan Mai Le, Tri-Nhan Nguyen
Keywords
Related Articles
- EP ID EP671031
- DOI 10.21276/ijlssr.2018.4.4.11
- Views 115
- Downloads 0
Pharmacological and Clinical Effects of Andrographis paniculata
Andrographis paniculata is an herbaceous plant has been effectively used in traditional Asian medicines for centuries. The demand for the herbal drug treatment of various ailments is increasing day by day and plant drugs...
Seasonal Variations in the Physicochemical Parameters of Ikpoba River Water Samples
Water is one of the most common natural resources that profoundly influence life. Water pollution occurs, when there is adverse change in the physical, chemical or biological condition of the water, which harmfully affec...
Integrated Management of Spodoptera litura: A Rewiew
Tobacco caterpillar, Spodoptera litura (Fabricius) is a widely distributed pest in South-East Asia, feeding on 63 plant species belonging to 22 families. It is a serious pest of soybean, pulses oilseeds, cotton and veget...
Association Between Socio-Behavioral Factors and Oral Health Status of 12-15 Year Old School children in Belagavi City- A Cross Sectional Study
Background- Oral health is a multi-factorial concept, determined by knowledge, behavior, and attitude of a person. Like any behavior carried out daily like a habit, oral health behaviors are also repeated like a habit. T...
Survey of Ethnobotanical Medicinal Plants Used by the People of District Guna, Madhya Pradesh, India
The documentation of the biodiversity and it is traditional as well as medicinal uses by the ethnic communities have assumed priority due to the misuse and rampant loss of biodiversity, the emerging threats of biopiracy...