Study of Biofilm Producing Property and Predisposing Factors in Bacterial Isolates from Community Acquired Urinary Tract Infections
Journal Title: INTERNATIONAL JOURNAL OF RECENT TRENDS IN SCIENCE AND TECHNOLOGY - Year 2014, Vol 9, Issue 3
Abstract
Introduction: Bacteria with biofilm producing property[BFPP] are associated with chronic and intractable urinary tract infections(UTI),especially with indwelling urinary catheter. Limited data on bacteria with BFPP in community acquired UTI[c-UTI] necessitated the present study. Aims and Objectives: To determine prevalence of biofilm producing property, predisposing risk factors and multi-drug resistance in bacterial pathogens from c-UTI. Material and Methods: Mid-stream urine specimens from75 patients, attending different OPDs were collected and processed by standard laboratory procedures. Bacterial isolates were tested for BFPP by Tube and Congo red dye tests. Predisposing risk factors and multi-drug resistance by interim guidelines incorporating CDC, EUCAST and FDA criteria were determined. Statistical analysis was done by Fisher’s exact test. Results: Prevalence of bacterial pathogens with BFPP from c-UTI was 83.75% (67/80). BFPP by Tube method alone, Congo red dye method alone and by both methods was 26.87% (18/67), 28.36% (19/67) and 44.78% (30/67) respectively. Prevalence of BFPP in E.coli, Klebsiella spp., Pseudomonas aeruginosa, Staphylococcus aureus and CONS was 96.43% (27/28), 78.95% (15/19), 85.71% (6/7), 77.78%(14/18) and 66.67%(4/6) respectively. Age, sex, department wise distribution, resistance for individual antibiotics and multi-drug resistance [53.75% (43/80) vs. 8.75%(7/80)] among bacteria with BFPP and non-BFPP was not statistically significant. Association of predisposing risk factors among isolates with BFPP was observed in only 24.61% (16/65) patients. Conclusions: Tube test and Congo red agar tests should be used together as screening tests to detect BFPP in bacterial isolates from c-UTI. BFPP was observed as natural property of bacteria rather than a virulence factor. No significant difference in multi-drug resistance observed among bacteria with BFPP and non-BFPP. Predisposing risk factors do not play role in acquisition or expression of BFPP in bacterial isolates from c-UTI.
Authors and Affiliations
Vishwajeet Bardoloi , Yogeesha Babu K. V , Basavarajappa K. G
Keywords
Related Articles
- EP ID EP136725
- DOI -
- Views 118
- Downloads 0
Evaluation of lumbar transforaminal epidural steroid injection by infraneural route
Conservative treatment of Lumbosacral radiculopathy mainly includes Transforaminal epidural steroid injection which delivers the inject ate directly to anterior epidural space, thus giving the nerve root a better relief...
Screening of Antibacterial and Antifungal Activity of Gandhaka Rasayana- an Ayurvedic Formulation
Introduction: Antimicrobial agents are commonly used nowadays for treating various bacterial and fungal infections. But still most of the physicians are unable to treat these infections appropriately due to hindrances li...
Study of hematological parameters in patients with diabetic retinopathy
Background: Diabetic Retinopathy (DR) is the major cause of blindness among the working population and is the most common microvascular complication of diabetes. Diabetes Mellitus (DM) is a complex disease characterized...
Association of IL-6 with Diabetes Mellitus in Indian Population from Navi Mumbai
Diabetes has already reached an epidemic level in India. Chronic inflammation has been considered as a culprit for setting pathophysiological changes which results in insulin resistance and type 2 diabetes mellitus. IL-6...
Study of influence of thyroid dysfunction on lipid profile
Introduction: Thyroid function regulates a wide array of metabolic parameters. Thyroid function significantly affects lipoprotein metabolism as well as some cardiovascular disease (CVD) risk factors, thus influencing ove...