Study of invitro antioxidant, antidiabetic and antibacterial activity of Clausena anisata (Willd). Hook (Rutaceae) leaf extracts
Journal Title: International Journal of Research in Pharmacology & Pharmacotherapeutics (IJRPP) - Year 2014, Vol 3, Issue 2
Abstract
Clausena anisata (Willd.) Hook of family Rutaceae, is a deciduous shrub or small tree that contains a wide array of compounds exhibiting diverse range of bioactivity. Leaf extracts are traditionallly used as effective remedies for worms infections, respiratory ailments, hypertension, malaria, fever, rheumatism, arthritis and other inflammatory conditions, headaches, pains, toothaches, convulsions. It has antimicrobial, fungicidal, insecticidal, and antidiabetic activities. The present study has been undertaken to find out the phytochemical, antioxidant, antidiabetic, and antibacterial activity of the leaf extracts of Clausena anisata (Willd.) Hook. Leaf was subjected to successive extraction using aqueous, alcohol, acetone, ethylacetate+chloroform and hexane to obtain the respective extracts. DPPH free radical Scavenging method was used for antioxidant activity test. Qualitative phytochemical analysis of leaf extracts of Clausena anisata (Willd.) Hook showed the presence of alkaloids, flavanoids, carbohydrates, saponnin, tannin, coumarin, proteins and aminoacids. Highest Scavenging was observed with leaf extracts of ethylacetate+chloroform (74.1%), acetone (74%), aqueous (70.8%) followed by hexane (67.1%) and alcohol (55.1%) at 100mg/ml. Antibacterial activity of ethylacetate+Chloroform extract was higher for cornybacterium and Proteus with 43mm zone of inhibition and for S.epidermis with 32mm zone of inhibition. It was found that the percentage increase of the rate of uptake of glucose into yeast cells was linear in different glucose concentrations used. The aqueous and alcohol leaf extracts of Clausena anisata (Willd.) Hook showed the maximum increase in 10mM glucose concentration i.e. 95% and 95.3% at 2000 µg/ml. The alcohol, aqueous and acetone extracts of Clausena anisata (Willd.) Hook showed appreciable (>20%) enzyme inhibitory activity against human urinary alpha amylase followed by hexane (3.4%) and ethylacetate+chloroform extracts (8.1%).
Authors and Affiliations
Arsia Tarnam
Keywords
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