The Effects of Enamel Matrix Proteins and Dentin Collagen on the Attachment of Periodontal Ligament Cells
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 14, Issue 6
Abstract
Background: The aim of this study was to evaluate the effect of enamel matrix proteins (EMP) and dentin collagen on the attachment of periodontal ligament cells to the root surfaces. Methods: Eighty-four root slices were obtained from forty-two lower anterior teeth that had been extracted due to the periodontal disease. The root slices were subjected to one of the following treatments: 1) control group 2) EDTA demineralization + EMP, 3) dentin collagen, 4) EDTA demineralization + dentin collagen. Periodontal ligament (PDL) cells (105/ml) were seeded and incubated for two hours on surfaces of the roots in each group. Following the incubation the numbers of the attached cells were calculated by colorimetric assay and the morphologies of the cells were evaluated by scanning electron microscopy (SEM). One-way ANOVA was used for statistical analysis (p=0.05). Results: No significant difference was found among the groups regarding the number of attached cells (p>0.05). However, the mean number of the attached cells was highest in the 2nd Group (EDTA demineralization + EMP), while it was lowest in Group 3 (dentin collagen). SEM evaluation of the dentin specimens revealed that the EMPs treated specimens exhibited elongated fibroblasts with filopodial extensions while the cells in the control and dentin collagen treated groups were round with thin and short filopodia. Conclusion: In this study, the EMPs were found effective in the attachment of cells on the root surface when compared to dentin collagen.
Authors and Affiliations
Sema Becerik, Şule Sönmez, Bilge Hakan Şen, Evren Evrenosoğlu, Ismet Deliloğlu Gürhan
Keywords
Related Articles
- EP ID EP340357
- DOI 10.9734/BJMMR/2016/24584
- Views 77
- Downloads 0
Salvigenin has Potential to Ameliorate Streptozotocin-induced Diabetes Mellitus and Heart Complications in Rats
Objectives: Flavonoids are the active ingredients, found in herbal remedies for amelioration the variety of disorders. Salvigenin is a plant flavenoid, which is found in Salvia officinalis. Salvigenin has an antioxidant,...
Prevalence and Antibiotic Susceptibility Pattern of Pathogens Isolated from Post-Operative Wounds of Diabetes Mellitus Patients in Uyo, Nigeria
This study was aimed at investigating the prevalence and antibiotic susceptibility of isolates from wounds of diabetic and non diabetic patients in University of Uyo metropolis. Eighty five wound swab samples were collec...
Pyogenic Granuloma: An Unusual Presentation
Pyogenic granuloma is one of the inflammatory hyperplasia seen in the oral cavity. It arises in response to various stimuli such as low-grade local irritation, traumatic injury, or hormonal factors. It is most commonly s...
Prevalence of Nosocomial Infections in Hebron-Palestine Hospitals
Background: Nosocomial infections, especially urinary tract infections, form a serious problem in hospitals, and are associated with increased mortality, morbidity, and prolonged hospital stay. In addition, the most infe...
Hepatocellular Carcinoma in Cirrhotic Liver: New Perspectives in Diagnostic Imaging
Hepatocellular carcinoma (HCC) is the second most common cause of cancer-relared death in the world. It incidence is expected to increase in the next decades. It is incrasingly being detected at an earlier stage, owing t...