The Expression of B7-H3 Molecule in Periodontal Disease
Journal Title: Journal of Dentistry, Shiraz University of Medical Sciences - Year 2017, Vol 18, Issue 1
Abstract
Statement of the Problem: T cells have been shown to play a role in the etiopathogenesis of periodontal disease. B7-H3, a costimulatory molecule, is found to be associated with regulation of T cell function in some tumoral tissues, as well as autoimmune and inflammatory diseases. Purpose: The aim of this study was to investigate the expression of B7-H3 molecule in healthy and diseased gingival tissue samples. Materials and Method: Gingival samples were taken from 2 groups (A and B) representing periodontal health and periodontal disease, respectively. These were paraffinized and processed to carry out immunostaining to identify B7-H3 expression. The slides were then examined under light microscope to assess the positive staining in epithelium and connective tissue. The intensity of positive staining in epithelium and the number of positive cells in the connective tissue were evaluated. Statistical analysis was done using kappa analysis and independent t-test. Results: Kappa analysis revealed good inter-examiner agreement for both the groups (group A: 0.718; group B: 0.797). Intensity of staining in the epithelium ranged from intense to moderate for both the groups. In the connective tissue, there was a statistically insignificant decrease (p value= 0.415) in the number of positive cells from group A (mean labeling index: 50.28±26.09) to group B (mean labeling index= 41.37±25.29). Conclusion: B7-H3 molecule has been found to be expressed in gingival tissue samples; however, it showed a statistically insignificant decrease in periodontal disease group compared to healthy group.
Authors and Affiliations
Saumya John, Arun Kurumathur, Avaneendra Talwar, Keerthana Kumar, Teenu Abraham, Ananthi Alagiri, Gnanasagar Walaja, Jasmine Clements
Keywords
Related Articles
- EP ID EP235182
- DOI -
- Views 108
- Downloads 0
Periodontal Condition in Patients with Rheumatoid Arthritis: Effect of Anti-rheumatic Drugs
Statement of the Problem: Rheumatoid arthritis and periodontitis are chronic inflammatory diseases with a possible bidirectional relationship. This link may be affected by many factors like drug consumption. Purpose: T...
Factors Affecting Dental Caries of Preschool Children in Shiraz, 2014
Statement of the Problem: Dental caries, the most common infectious disease, can lead to several consequences, including inflammation and bleeding of the gum, abscess formation, tooth loss, and subsequently loss of avail...
Effect of Beta-Blockers on Number of Osteoblasts and Osteoclasts in Alveolar Socket Following Tooth Extraction in Wistar Rats
Statement of the Problem: Various researchers have suggested the use of β2-adrenergic receptor antagonists in prevention or treatment of bone resorption. Purpose: This study aimed to evaluate the effect of β2-adrenergic...
Comparative Evaluation of Penetration Ability of Three Pit and Fissure Sealants and Their Relationship with Fissure Patterns
Statement of the Problem: Pit and fissure sealant placement is considered as an effective modality for prevention of caries on occlusal surfaces. Penetration, retention and lateral wall adaptation are the key factors in...
Intra- Socket Transplantation of Bicuspid to Re-establish Biologic Width
Transplantation and reposition of a tooth in its socket is relatively simple and predictable and can be considered as an alternative treatment option particularly in patients with financial limitation. This report descr...