The Various Fixatives Effects on Lymph Nodes – A Histological Study
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2018, Vol 6, Issue 1
Abstract
The fixation also in optical differentiation of cells and tissue constituents by altering their refractive indices in varying degrees. The fixation is a critical step in the preparation of histological sections by which biological tissues are preserved from decay, thereby preventing autolysis or putrefaction. This is of value in the microscopic examination of cells and tissue. The present study aimed to find the best fixative for a particular organ, so that the best histological section details can be produced. We studied the effect of five different types of fixatives. An essential part of all histological and cytological techniques is preservation of cells and tissues as they naturally occur. To accomplish this, tissue blocks, sections or smears are usually immersed in a fixative fluid, although in the case of smears, merely drying the preparation acts as a form of preservation. The aim of the current study is to see the effect of the following fixatives namely 10% formalin, Buffered 10% formalin, Bouin’s fluid, Zenker’s fluid, Carnoy’s fluid on liver tissues and to observe the optimum result in a particular fixative in H&E sections. There is no single fixative which can be considered as best fixative for all purposes. Best fixatives for architectural preservation nare Carnoy’s fluid and Zenker’s fluid. Best fixative for study of nuclear details is Bouin’s fluid.
Authors and Affiliations
Dr. Ved Prakash, Dr. Muktyaz Hussein
Keywords
Related Articles
- EP ID EP474754
- DOI -
- Views 166
- Downloads 0
The Role of Chest X-ray in the Diagnosis of Pneumonia
Pneumonia is an inflammatory condition of the lung affecting primarily the microscopic air sacs known as alveoli. It is usually caused by infection with viruses or bacteria and less commonly other micro-organisms, certai...
Neostigmine as an Adjuvant to Epidural Anaesthesia With 0.5% Bupivacaine for Vaginal Hysterectomy: A Comparative Study
Various drugs have been used as epidural adjuvants to enhance the quality and duration of surgical anaesthesia. We aimed at evaluating the effects of Neostigmine, an acetylcholine esterase inhibitor, as an adjuvant to ep...
A Study of the Effects of Formalin on First Year MBBS Studen
Formalin has been used as a preservative for cadaver in anatomy for years now. 1st year MBBS students are not accustomed to this chemical earlier and have various reactions when they come in contact with this. Therefore...
Study of Somatotypes in students of western Maharashtra Population by Anthropometric Method
Somatotype though a method of describing various human forms in three numbers. It gives rough idea about the physique of the individual and variation in muscle and skeletal mass. The study was done to evaluate the studen...
Tooth or Odontoma - A Clinical Dilemma
It has been proposed, in an animal model, that it is possible to cause disintegration of a developing tooth bud to such an extent, that it leads to the formation of an odontome. However, such an occurrence in the human d...