THERMOSTABLE RESTRICTION ENDONUCLEASES FROM THERMOPHILIC BACTERIA
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2014, Vol 5, Issue 4
Abstract
Restriction endonucleases are not only important for bacteria and archaea as their defense mechanism but they are considered as foundation of recombinant DNA technology. The whole empire of biotechnology depends upon type II restriction endonucleases isolated from the diversified array of bacteria. There are 4,096 known restriction enzymes, including 106 Type I, 3, 950 Type II, 21 Type III and 19 Type IV restriction enzymes. Among which 646 type II enzymes have been cloned and 611 have been sequenced. A number of restriction endonucleases have been isolated from thermophilic bacteria also, having higher thermal stability and tolerance to most of the chemical denaturants. In Restriction Enzyme Database, about 600 type II restriction enzymes have been listed showing growth temperature above 400C. Nowadays these enzymes have found their importance in isolating mutant strains and for production of antisense oligonucleotides. Thermostable restrictions enzymes are also applied as important tool for molecular biology, industrial works and in cellular processes.
Authors and Affiliations
Dipali Gupta , Nevadita Sharma
Keywords
Related Articles
- EP ID EP94097
- DOI 10.7897/2230-8407.050455
- Views 104
- Downloads 0
CONCEPTUAL STUDY OF THE EFFECT OF ‘UTTARBASTI’ IN ‘VANDHYATVA’ WITH SPECIAL REFERENCE TO CERVICAL FACTOR OF INFERTILITY
The most disappointing feeling for a woman is, if she could not be a mother. The present day’s life style of women is very stress full, unhealthy and unhygienic due to unawareness, hectic and fast running schedule. Vandh...
A NOVEL APPROCH: PULSATILE DRUG DELIVERY SYSTEM
Pulsatile drug delivery system (PDDS) is useful in the treatment of disease, in which drug availability is timed to match rhythms of disease, in order to optimize therapeutic effect and minimize side effects. PDDS have a...
PHARMACOGNOSTICAL STANDARDIZATION AND ANTIMICROBIAL ACTIVITY OF LEAVES OF SYZYGIUM CUMINI (LINN.) FROM VARIOUS REGION OF NORTH INDIA
Syzygium cumini is one of the widely used medicinal plants in the treatment of various diseases in particular diabetes. In present investigation, the pharmacognostic study of Syzygium cumini leaf is carried out from diff...
TRANSFEROSOME: AN OPPORTUNISTIC CARRIER FOR TRANSDERMAL DRUG DELIVERY SYSTEM
Transdermal route of drug delivery system has received a great attention in pharmaceutical research and it has already proved its superiority in various respects than oral route, which has a number of problems in drug de...
PREPARATION AND IN-VITRO EVALUATION OF BACLOFEN LOADED MICROSPHERES
The present study was aimed to prepare Baclofen microspheres for sustained release using various polymers such as ethyl cellulose (hydrophobic), hydroxy propyl methyl cellulose (hydrophilic) by employing solvent evaporat...