TP53 Gene Alterations and P53 Protein Accumulation in Human Esophageal Cancer in Khartoum State, Sudan.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 11
Abstract
TP53 gene alterations were screened in 50 formalin fixed paraffin embedded (FFPE) human esophageal cancer (EC) specimens comprising 43 squamous cell carcinoma (SCC) and 7 adenocarcinoma (AC) using immunohistochemistry (IHC) and conventional PCR followed by DNA sequencing to detect p53 accumulation and mutation, respectively, in Khartoum state, Sudan. TP53 gene mutations were detected in 20 (40%) of the 50 EC cases investigated. Five out of 13 (38.5%) of SCC cases that stained positive for p53 protein, showed TP53 mutations, only one (14.3%) of seven AC samples was positive for p53 protein without associated mutation and 4/7 (57.1%) showed TP53 mutations only. Immunohistochemical stain was positive in 14/50 (28%) of EC samples; 13 (92.9%) of them were SSC cases and one (7.1%) AC. Five out of forty three (11.6%) of SSC and none of AC cases showed both immunohistochemical positive stain and mutation of TP53 gene while 8/43 (18.6%) of SSC and 1/7 (14.3%) of AC samples showed immunohistochemical positive stain but no TP53 gene mutation were present. Finally 15/50 (30%) of EC, 11 (73.3%) were SSC and 4 (26.7 %) AC showed immunohistochemical negative stain and positive TP53 gene mutations. Immunohistochemical patterns of p53 were not related significantly to mutational analysis results in the cases examined. Mutation analysis is recommended to be done on the entire length of the gene using fresh tissue to complete the whole picture in future studies.
Authors and Affiliations
Rihab M. Elfaki, Mohammed S. AbdelAziz, Hisham N. Eltayb, Munsoor M. Munsoor, Ahmed A. Gameel
Keywords
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- EP ID EP416496
- DOI 10.9790/0853-1711086267.
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