Use of abscisic acid, mannitol and the reduction of the concentration of the salts of the culture medium in the in vitro conservation of Ipomoea batatas
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 1
Abstract
Nodal segment from in vitro plants of Jewel clone sweet potato were culture in the following culture medias: a) Murashige and Skoog (1962) salts supplemented with abscisic acid (0.0, 1.0, 5.0 y 10.0 mg.l-1), b) Murashige and Skoog (1962) salts supplemented with mannitol (0.0, 10.0 15.0 y 20.0 g.l-1) and c) different Murashige and Skoog (1962) salts concentration (100, 75, 50 and 25%) were used. Survival during conservation was high in all experiment. The 5.0 and 10.0 mg.l-1 abscisic acid strongly inhibited the plants growth. It was reached a decrease of growth when using mannitol in the culture medium. The media with 25% and 50% MS salts concentration caused a low of plants growth. When cultured in fresh culture medium it was achieved a high recovery ratio of conserved material.
Authors and Affiliations
Angel Espinosa Reyes, Leonardo Salas Hernández, Orlando González Paneque, Juan J. Silva Pupo
Keywords
Related Articles
- EP ID EP697784
- DOI -
- Views 55
- Downloads 0
Standardization of a protocol for obtaining genomic DNA for the quantification of 5mC in epicormic outbreaks of Tectone grandis L.
The present investigation was carried out with the objective of defining an extraction and purification method that it provided deoxyribonucleic acid (DNA) appropriate to determine the percentage of 5mC in the genomic DN...
Alternatives of in vitro establishment in the micropropagation of the Guana
Alternative of the in vitro establishment in the micropropagation of the Guana. An alternative that should be exploited in forest species, to achieve high volums of plants and to establish improved plantations, is the em...
Use of Gentamicine sulfate for the control of Pantoea agglomerans, contaminant of the in vitro multiplication of Solanumtuberosum L cv. Desiree
Bacterial contamination is one of the principal problems of plant tissue culture. The use of natural or synthetic antimicrobial substances represent an alternative for it solution. In potato micropropagation bacterial...
Use of abscisic acid, mannitol and the reduction of the concentration of the salts of the culture medium in the in vitro conservation of Ipomoea batatas
Nodal segment from in vitro plants of Jewel clone sweet potato were culture in the following culture medias: a) Murashige and Skoog (1962) salts supplemented with abscisic acid (0.0, 1.0, 5.0 y 10.0 mg.l-1), b) Murashige...
Use of AFLP markers for molecular characterization of two crops with agricultural interest
The AFLP technique is based on the selective amplification of restriction fragment by polymerase chain reaction (PCR) and consists of four basic steps; digestion with restriction enzymes of deoxyribonucleic acid (DNA),...