Use of tissue culture mutagenesis in vitro for the improvement of resistance to Alternaria solani in potato var. ̈Desirée ̈
Journal Title: Biotecnología Vegetal - Year 2001, Vol 1, Issue 1
Abstract
Two phases of work were carried out with the objective of obtaining somaclons in Irish Potato (Solanum tuberosum L.) that present a superior behavior with respect to the variety Desirée against the early blight disease caused by Alternaria solani (Sor.) and A. alternata (Fr.) Keissler. In the first phase, the aspects related to the pathogen, the incubation time and the method of application of the filtered culture on the vitroplants were studied the immersion of the roots as the effective method of inoculation. The biological effectiveness of the different concentrations of the filtrate on the vitroplants of the variety Desirée (susceptible) and the specie Solanum chacoense (resistant) was checked, taking the dilution of 1:3 v/v as the effective for the selection. A scale was elaborated to evaluate the intensity of the damages caused by the filtrate and a selection scheme was established for the genetic improvement of the variety Desirée. The second phase had as its objective the massive inoculation with the filtered culture of the fungus 1 000 somaclons were obtained from the irradiated calli with gamma radiation. Forty-five somaclons presented levels of affectation inferior to that of the original variety. The behavior of the somaclons selected in vitro was evaluated in the greenhouse against the disease and six somaclons presented levels of affectation inferior to that of the original variety.
Authors and Affiliations
Novisel Veitía, Miguel Angel Dita, Lourdes García, Lidcay Herrera, Idalmis Bermúdez, Mayra Acosta, Justo Clavero, Pedro Orellana, Carlos Romero, Leonardo García
Keywords
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