Validated UV spectrophotometric method for quantitative analysis of tramadol in bulk and pharmaceutical dosage form
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2016, Vol 4, Issue 1
Abstract
A precise, accurate, economical and simple UV spectrophotometric method has been developed for the determination of Tramadol hydrochloride in bulk and pharmaceutical dosage form. The Tramadol hydrochloride shows maximum absorbance at 273.5 nm in water and obeys Beer’s law in the concentration range of 10-50 μg /mL with a correlation coefficient (r2 = 0.9999). The results of analysis were validated by recovery studies. The percentage recovery method was found to be 99.53-100.41%. The relative standard deviation was found to be < 2.0 % in all cases. The Proposed spectrophotometric method was validated as per the ICH Q2 (R1) guidelines. The method was successfully feasible to pharmaceutical formulation because no chromatographic interferences from the tablet excipients were found. The proposed method was found to be simple, accurate and reliable for routine quantification of Tramadol hydrochloride in bulk form and pharmaceutical dosage forms.
Authors and Affiliations
Ch. Jhansi Lakshmi, Panchumarthy Ravisankar, K. V. S. Santosh Kumar, P. Srinivasa Babu
Keywords
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