Whole Genome Sequencing of Hepatitis B Virus (HBV) Strains from Myanmar
Journal Title: JOURNAL OF BIOLOGICAL ENGINEERING RESEARCH AND REVIEW - Year 2017, Vol 4, Issue 2
Abstract
Hepatitis B virus (HBV) is an important etiological agent of acute or chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. It is estimated that there are currently more than 350 million chronically infected people worldwide and this poses major health problems worldwide, especially in Asian Pacific countries. To date, 10 HBV genotypes, scattered across different geographical regions, have been identified. Complete genome sequences are more reliable for the analysis of genotype and sub-genotyping. In addition to this, certain mutations which may affect the diagnostic detection, drug resistance, disease progression and effective vaccination of HBV can also be detected. In this study we successfully sequenced the 15 full length genetic sequences (3.2kbp) of HBV isolates from HBV infected Myanmar peoples. Genotyping was done by using HepSEQ web based program, and drug resistant mutations were detected by geno2pheno web based analytical tools. All 15 sequences revealed genotype C and no drug resistant mutations were seen among these isolates. There was also no known vaccine escape mutation among these isolates. This is the first time to sequence the whole genome of Myanmar HBV isolates by Department of Medical Research, and these whole genome sequences will act as baseline data for further genetic studies regarding HBV.
Authors and Affiliations
Aung Zaw Latt, Nyi Nyi Win, Kay Thi Aye, Hlaing Myat Thu, Yi Yi Kyaw, Kyaw Zin Thant
Keywords
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