ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF LEVOSULPIRIDE AND PANTOPRAZOLE IN TABLETS BY RP-HPLC METHOD
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 3
Abstract
A simple, precise, accurate, rapid and economical reverse phase high-pressure liquid chromatographic method has been developed as per ICH norms for the simultaneous estimation of Levosulpride and pantoprazole sodium from pharmaceutical formulation. The method was carried out on a Column – C18 (250mm x 4.6mm x5 μ) with a mobile phase consisting of buffer (adjusted to pH6.8 with 1% triethylamine): Acetonitrile (65:35v/v) and filtered through 0.45μ cellulose nitrate filters. The flow rate 1.0mL/min. Detection was carried out at 280 nm. The retention time of LVS and PNT was 2.41 and 6.86 min respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification and solution stability. The proposed method can be used for the estimation of these drugs in combined dosage forms.
Authors and Affiliations
Kalaiselvi P. | Asistant Professor, Department of pharmaceutical chemistry, JKKMMRF College of pharmacy, B. Komarapalayam, Tamil Nadu, India, K. G. Lalitha*| Professor, Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai, Tamil Nadu, India
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