ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF LEVOSULPIRIDE AND PANTOPRAZOLE IN TABLETS BY RP-HPLC METHOD
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 3
Abstract
A simple, precise, accurate, rapid and economical reverse phase high-pressure liquid chromatographic method has been developed as per ICH norms for the simultaneous estimation of Levosulpride and pantoprazole sodium from pharmaceutical formulation. The method was carried out on a Column – C18 (250mm x 4.6mm x5 μ) with a mobile phase consisting of buffer (adjusted to pH6.8 with 1% triethylamine): Acetonitrile (65:35v/v) and filtered through 0.45μ cellulose nitrate filters. The flow rate 1.0mL/min. Detection was carried out at 280 nm. The retention time of LVS and PNT was 2.41 and 6.86 min respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification and solution stability. The proposed method can be used for the estimation of these drugs in combined dosage forms.
Authors and Affiliations
Kalaiselvi P. | Asistant Professor, Department of pharmaceutical chemistry, JKKMMRF College of pharmacy, B. Komarapalayam, Tamil Nadu, India, K. G. Lalitha*| Professor, Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai, Tamil Nadu, India
Keywords
Related Articles
- EP ID EP2534
- DOI -
- Views 369
- Downloads 23
The Comparsion of Piperacillin / Tazobactem with Clindamycin in Diabetic Foot Ulcers
To evaluate and compare the study and efficacy of piperacillin/tazobactem with clindamycin in diabetic foot ulcer according to Wagner’s different grades of classification of diabetic foot ulcer. The study was conducted...
QUANTIFICATION OF HBV DNA AND ITS IMPACT ON VIRAL LOAD MONITORING
HBV is a causative agent for chronic hepatitis, and can cause liver cirrhosis and hepatocellular carcinoma. Chronic carriers are at high risk of long term complications of infection, including chronic hepatitis, cirrhos...
SAFRANAL AMELIORATES SODIUM VALPROATE-INDUCED LIVER TOXICITY IN RATS BY TARGETING GENE EXPRESSION, OXIDATIVE STRESS AND APOPTOSIS.
Sodium valproate (VPA) is a potentially hepatotoxic antiepileptic drug. Safranal(SAF), an essential oil derived from saffron, possesses a potent antioxidant, anti-apoptotic and anticancer properties. The current study ai...
Study of demographic factor relationship with the drug abusing habits of students in Bikaner zone of Rajasthan state India
India has unique cultural diversity. Diversity in culture influences various sociological impacts. Present study help to identify the impact of demographic factors including gender, cast, family type, background, food ha...
Preliminary Phyto-chemical screening of Abelmoschusesculentus Linn.
Herbal drugs with little or no adverse effects are becoming a necessity in our daily life. The herbal products & herbal drug extracts are now a day’s being used extensively for the preparation of pharmaceutical dosage fo...