Callus formation of Persea americana Mill. Catalina cultivar starting from in vitro plant leaf segments
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 2
Abstract
ABSTRACT The importance of establishing protocols for the regeneration of fruit-trees through out the organogenesis and the somatic embryogenesis is based on the possibility to use them in the propagation of the species or in programs of genetic improvement. Specifically the Catalina cultivar is very coveted in Cuba, however it has not been introduced in vitro. The present work had the objective of forming callus starting from leaves of in vitro plants from avocado tree of the Catalina cultivar. The phase of multiplication of the in vitro plants was carried out in temporary immersion systems in order to favour the expansion of the leaves. Erlenmeyers 1.0l were used In the TIS and immersions every four and eight hours with a minute of duration were carried out. The effect of the Picloram, the 6 BAP and the 2,4-D on leaves of in vitro plants for the formation of callus was studied. The plants with more quality were obtained when immersions every 8 hours were carried out. It was also possible to form callus using sections of leaves of in vitro plants as explants in a culture medium with 0.1 Picloram mgl -1. Key words
Authors and Affiliations
Marisol Freire Seijo, Alba Patricia Balsero Fiquitiva, Rafael Gómez Kosky, Yudith García Ramírez, Maite Chávez
Keywords
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