Callus formation of Persea americana Mill. Catalina cultivar starting from in vitro plant leaf segments
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 2
Abstract
ABSTRACT The importance of establishing protocols for the regeneration of fruit-trees through out the organogenesis and the somatic embryogenesis is based on the possibility to use them in the propagation of the species or in programs of genetic improvement. Specifically the Catalina cultivar is very coveted in Cuba, however it has not been introduced in vitro. The present work had the objective of forming callus starting from leaves of in vitro plants from avocado tree of the Catalina cultivar. The phase of multiplication of the in vitro plants was carried out in temporary immersion systems in order to favour the expansion of the leaves. Erlenmeyers 1.0l were used In the TIS and immersions every four and eight hours with a minute of duration were carried out. The effect of the Picloram, the 6 BAP and the 2,4-D on leaves of in vitro plants for the formation of callus was studied. The plants with more quality were obtained when immersions every 8 hours were carried out. It was also possible to form callus using sections of leaves of in vitro plants as explants in a culture medium with 0.1 Picloram mgl -1. Key words
Authors and Affiliations
Marisol Freire Seijo, Alba Patricia Balsero Fiquitiva, Rafael Gómez Kosky, Yudith García Ramírez, Maite Chávez
Keywords
Related Articles
- EP ID EP684319
- DOI -
- Views 136
- Downloads 0
Determination of genetic stability of in vitro propagated plants of Vasconcellea x helbornii Badillo (Badillo) using ISSR markers
The mountain papayuelo, among them babaco [Vasconcellea x helbornii Badillo (Badillo)], are native of Ecuador and has a great nutritional value, that is it has a huge national and international demand. The objective of...
In vitro establishment of Handroanthus heptaphyllus nodal segments
Micropropagation could be used as a viable alternative in the mass production of Handroanthus heptaphyllus (Vell.) Mattos (white lapacho). The objective of the present work was to establish in vitro of nodal segments of...
Detection of biochemical compounds related to defensive response in Musa spp. plants inoculated artificially with Mycosphaerella fijiensis
Knowledge about biochemical events involved in both compatible and incompatible interaction in Musa-M. fijiensis pathosystem it is still very limited. Then, the use of tools to study fast and simply plant defence mecha...
Introduction to the immune system in plants
Many plants are invaded by pathogenic microbes that worsen their growth and reproduction. Plants have a defense system that goes from physical barriers to molecular and systemic signals, similar to the animal innate immu...
Evaluation of Pectimorf as a growth regulator in the formation of callus with embryogenic structures in Ipomoea batatas
Tuberous roots of sweet potato belonging to the clone INIVIT B 93-1, were gather ad and placed in flasks with water in the laboratory to induce the emergence of the yolks. Later on, explants of the limbo was selected to...