Detection of antibacterial activity of Querus infectoria against MRSA isolates
Journal Title: Journal of Pharmaceutical and Biomedical Sciences - Year 2012, Vol 2, Issue 8
Abstract
Abstract: Background:- Methicillin-resistant Staphylococcus aureus (MRSA) has been well-documented as a major cause of hospital infections. The aim of the present work was to evaluate the antibacterial potential of aqueous and ethanol extracts of nutgalls of Quercus infectoria by disc diffusion method, determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values. Materials and Methods: A total of 103 clinical isolates of Staphylococcus aureus were collected from Tirunelveli Medical College Hospital. The isolated strains of S. aureus were subjected to the following phenotypic methods viz., Oxacillin screen agar and Cefoxitin disc diffusion test to screen and confirm MRSA. The nutgalls of Quercus infectoria were extracted with 95% ethanol and water. The antibacterial activity of aqueous and ethanol extract of Quercus infectoria was determined against Staphylococcus aureus ATCC 25923, MRSA ATCC 43300 and clinical isolates by disc diffusion method, MIC and MBC. The MIC of the extracts were then determined using the twofold serial micro dilution technique at a concentration ranging from 50 mg/ml to 0.4 mg/ml. The MBC values were finally obtained from the MIC micro titre wells which showed no turbidity after 24 hrs of incubation by sub culturing method. Results: Ethanolic and aqueous extracts of Quercus infectoria demonstrated significant antibacterial activities against all strains of methicillin-resistant Staphylococcus aureus (MRSA). Inhibition zones of ethanolic and aqueous extracts of Quercus infectoria for clinical isolates of MRSA strains were in the range 11-17 mm. Staphylococcus aureus ATCC 25923, MRSA ATCC 43300 demonstrated similar results. The MIC values of aqueous extract for Staphylococcus aureus ATCC 25923, MRSA ATCC 43300 and clinical isolates of MRSA strains were 0.4-1.6 mg/ml, 0.4-1.6 mg/ml and 0.4-3.2 mg/ml respectively and minimum bactericidal concentration (MBC) values were 3.2 mg/ml, 6.3 mg/ml and 6.3 mg/ml, respectively. The MIC values of ethanolic extract for Staphylococcus aureus ATCC 25923, MRSA ATCC 43300 and clinical isolates of MRSA strains were 0.4-1.6 mg/ml, 0.4-0.8 mg/ml and 0.4-3.2 mg/ml respectively and MBC values were 0.8-1.6 mg/ml, 0.4 mg/ml and 3.2- 6.3 mg/ml respectively. Conclusion: The present study demonstrated that the ethanolic extract of Quercus infectoria had significant antibacterial activities against all strains of Methicillin Resistant Staphylococcus aureus (MRSA). This finding provides an insight into the usage of the nutgalls of Q. infectoria in traditional treatment of wounds or burns associated with MRSA infections
Authors and Affiliations
G. Sucilathangam, S. Nithya Gomatheswari, C. Pauline Vincent, N. Palaniappan
Keywords
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