Effect of Sodium Chloride on the in vitro development of cultivars 'Gran enano' and 'FHIA 03'
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 2
Abstract
The effect of Sodium Chloride levels on in vitro development of Gran Enano (AAA) and `FHIA 03`(AABB) cultivars was determined. Researches were carried out at Instituto de Investigaciones en Viandas Tropicales (INIVIT) from 1999 to 2001. Meristems (3 mm) were situated in M 3 medium supplented with different sodium chloride concentrations at a range of 0 10 000 ppm. They were incubated during 40 days in cach subculture until 80 days when they were evaluated at 25 ± 27°C temperature, DFFF 45 µmol m -2 s -1 light intensity a photoperiod of 16 hours per day and 85% relative humidity. Tolerance levels to sodium chloride are highly influenced by genotypes used. Gran Enano cultivar had success up to 4 500 ppm with a surviving of 96.7% and 25% damaged leaves. FHIA 03 Hybrid showed tolerance to high salt levels from 2 500 to 8 000 ppm with 80.9% surviving but leaves were affected at 72.5%. Studies are recommended to continue with target genotypes including main marketable clones and diploids for genetic improvement.
Authors and Affiliations
José de la C. Ventura, Luis Ruiz, Jorge López, Víctor Medero, Magaly García, Sergio Rodríguez, Manuel Cabrera, Marilys Milián, Nery Montano, Damisela Reynaldo, Marleny Torres, Dinorath Carvajal, Juan R. Gálvez, Jesús García, Julia Albert
Keywords
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