HPLC Simultaneous Determination of Khellin and Visnagin in Ammi visnaga L. Fruits
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
A rapid and accurate HPLC method was developed for determination of both Khellin and Visnagin in Ammi visnaga L. extracts. The calibration curve of Khellin was linear with correlation coefficient (r2 ) = 0.9990; over a concentration range of 53.60 – 355.00 mg/l; with a retention time of 9.19 minutes. While the calibration curve of Visnagin was linear with correlation coefficient (r2 ) = 0.9999; over a concentration range of 245.40 – 1533.75 mg/l; with a retention time of 10.77 minutes. The relative standard deviation (RSD) was found to be < 2. The proposed method was validated and successfully applied for simultaneous determination of Khellin and Visnagin in the extracts. The method described is quite suitable for routine analysis -Pyrones in Ammi visnaga L. extracts (prepared by traditional solvent extraction as well as supercritical fluid extraction).
Authors and Affiliations
Mokhtar M. Bishr, Mohamed A. El-Degwy, Mohamed A. Amin, Osama M. Salama
Keywords
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- EP ID EP387020
- DOI 10.9790/3008-110604110115
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