In vitro multiplication of Macleania rupestris (Kunth) A.C. Sm. (Ericaceae)
Journal Title: Biotecnología Vegetal - Year 2019, Vol 19, Issue 4
Abstract
Macleania rupestris (Kunth) A.C. Smith (Ericaceae) is a plant native to the Andes, which produces edible fruits with great food and commercial potential. M. rupestris plants have been in vitro established from seeds of ripe fruits and to increase their number it is necessary to define the conditions of their multiplication. The objective of the investigation was to determine the effect of zeatin and kinetin to in vitro multiply M. rupestris plants. As a basal culture medium, Woody Plant (WP) was used. Treatments included the CIRGEBB culture medium (WP + 0.5 mg l -1 zeatin), A (WP + 0.5 mg l -1 kinetin), B (WP + 1.0 mg l -1 kinetin) and WP as control. The variables evaluated were explant height (cm), number of shoots and number of leaves, at 60 and 120 days of culture. The use of zeatin and kinetin in the WP culture medium allow in vitro multiplication of M. rupestris. However, the addition of zeatin favors the development of plants with phenotypic characteristics suitable for micropropagation. Based on the results, it is proposed to use the culture medium A (WP + 0.5 mg l -1 kinetin).
Authors and Affiliations
Antonietta Ornella Gutiérrez Rosati, Yngrid De la Cruz Chacón
Keywords
Related Articles
- EP ID EP698047
- DOI -
- Views 59
- Downloads 0
Ochrobactrum anthropi, contaminants of in vitro culture of sugarcane cells and tissues
The purpose of this paper was to report, for first time, the presence of Ochrobactrum anthropi as contaminant of in vitro cell and tissue culture of sugarcane. Bacteria contaminant of in vitro plants were isolated direct...
Influence of the genotype and inoculation density on the differentiation of somatic embryos of Coffea arabica L. cv. Red Caturra and Coffea canephora cv. Robust
The conditions were established for the differentiation of somatic embryos from cell suspensions in the genotype Caturra rojo (Coffea arabica) and Robusta (Coffea canephora). Cell suspensions with high embryogenic potent...
Callus formation of sugar cane var. ‘SP 70-1284’ in liquid culture medium from field explants
Explants from immature sections of young leaves in the stems of the variety of sugarcane ‘SP 70-1284’, were taken directly of field whit the objets of study the capacity for organogenic callus formation. The effect of t...
Control of bacterial contamination in artificial sugarcane seed through early detection methods
The studies for stablishment of artificial seed technology in sugarcane (Saccharum spp hybrido) have not been free of damage produced by bacterial contaminations, principally in the stage before somatic embryos encapsula...
Basic aspects of in vitro organogenesis
Plant regeneration in vitro of Pinus genus by somatic embryogenesis has been long studied. However, the in v itro establishment of plants from apical shoots and in vitro multiplication of these shoots have been an alter...