Osmotic Fragility Index and Stability of Human Erythrocytes in the Presence of Four Oral Antiretroviral Drugs
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2015, Vol 3, Issue 2
Abstract
The relative stability of human erythrocyte membrane in the presence of four oral antiretroviral drugs was investigated in vitro. Blood collected by venipuncture from 60 confirmed healthy humans were used for the study. The osmotic fragility of the erythrocytes was determined by measuring the release of haemoglobin from blood added to tubes containing serially diluted phosphate buffered saline (PBS, pH 7.4). Results revealed that increase of mean corpuscular fragility was not significant (p>0.05) in the presence of nevirapine, lamivudine and abacavir sulfate, while, efavirenz cause a significant (p<0.05) increase from 0.49±0.01 to 0.53±0.04mg/100ml. The erythrocytes were stable at 0.2mg/ml of nevirapine; 0.6mg/ml efavirenz; and 0.2 and 0.4mg/ml lamivudine. Results show that all drugs have the capacity to increase the permeability of the erythrocyte membrane and can compromise the integrity of the erythrocyte. Keywords: Erythrocyte, Antiretroviral drugs, Nevirapine, Lamivudine, Abacavir sulfate, Efavirenz
Authors and Affiliations
Wodu, E. , Uwakwe . , A. A. , Monanu, M. O.
Keywords
Related Articles
- EP ID EP373398
- DOI -
- Views 84
- Downloads 0
Study of Pulse Wave Velocity and Ankle Brachial Index in different age-groups and their role in assessment of early atherosclerotic risk
A total of 240 subjects of different age groups were studied for detection of early atherosclerotic risk by measuring their Pulse Wave Velocity (PWV) and Ankle Brachial Index (ABI) with the help of Vascular Profiler (VP-...
Crisis Management of Perioperative Bronchospasm in a Neonate with Sepsis
Abstract:Bronchospasm in association with anaesthesia may appear as an entity in its own right or be a component of another problem such as anaphylaxis. It may present with expiratory wheeze, prolonged exhalation or, in...
Significance of Relationship between Platelet Indices and Hyperlipidemia in Predicting Ischaemic Events
Abstract: Atherosclerosis begins with damage to the endothelium. It’s caused by high blood pressure, smoking, or high cholesterol. That damage leads to the formation of plaque.When bad cholesterol, or LDL, crosses the da...
Role of Surfactant by INSURE Approach in Management of Preterms with Respiratory Distress Syndrome
Surfactant replacement therapy by ‘INSURE’ is a gentle ventilatory approach in preterms with Respiratory Distress Syndrome (RDS) reducing the mortality and is associated with less adverse sequelae than mechanical ventila...
Clinical Profile and Etiological Evaluation of Pancytopenia in a Tertiary Care Hospital
Abstract: Evaluation of clinical and etiological profile of Pancytopenia in a tertiary care hospital. This is a Retropective study conducted in dept of medicine in SDMCMSH Sattur Dharwad during the period of July 2017 –...