RP – HPLC Simultaneous Estimation of Diclofenac Diethylamine and Lidocaine in Pharmaceutical Gel Formulation
Journal Title: International Journal of Research in Pharmacy and Science - Year 2012, Vol 2, Issue 4
Abstract
A new specific, precise, accurate and robust RP-HPLC method has been developed for the simultaneous determination of Lidocaine and Diclofenac Diethylamine in a pharmaceutical gel formulation. The chromatographic separation was carried out at Jasco HPLC system consisting of Jasco PU 2075 pump having browin software.The stationary phase was PrincetonSPHER 100 C18 column (250mm X 4.6mm, 5µ).The mobile phase was Acetonitrile: Potassium dihydrogen phosphate (0.01M): Butane sulfonic acid sodium salt (45:55:0.1%), and adjust to pH 6.8± 0.05 with triethylamine. Detection was carried out at 261nm using Jasco UV 2075. The flow rate was 1.0ml/min and retention time was about 5.7min and 14.025min.for Diclofenac Diethylamine and Lidocaine respectively. The linearity was obtained in the concentration range of 20-300ug/ml and 50-500ug/ml for Lidocaine and Diclofenac Diethylamine respectively. Mean percentage recoveries were 99.84% for Lidocaine and 99.78% for Diclofenac Diethylamine. The LOD of Lidocaine and Diclofenac Diethylamine was found to be 20 µg/ml and 12.0 µg/ml whereas the LOQ was 35 µg/ml and 60 µg/ml respectively. The assay values of both the analytes was found to be well within the limits that is 101.5% and 98.25% for Lidocaine and diclofenac Diethylamine respectively. Percentage relative standard deviation of percent assay values for replicate sample preparation was 1.11% for Lidocaine and 0.95% for Diclofenac Diethylamine .The method was robust with respect to change in flow rate, and composition of mobile phase.
Authors and Affiliations
Asghar Suhail, Sheikh Safeena, Showkat Patni
Keywords
Related Articles
- EP ID EP103740
- DOI -
- Views 148
- Downloads 0
Formulation and Evaluation of Gastroretentive Floating Tablet of Aceclofenac
Tablet can be Utilized for precise delivery of drugs and reduce the drug concentrations at sites other than the target organ. The present study performed by preparation and evaluation of floating tablets of Aceclofenac a...
RP – HPLC Simultaneous Estimation of Diclofenac Diethylamine and Lidocaine in Pharmaceutical Gel Formulation
A new specific, precise, accurate and robust RP-HPLC method has been developed for the simultaneous determination of Lidocaine and Diclofenac Diethylamine in a pharmaceutical gel formulation. The chromatographic sepa...
ADVANCE ASPECTS OF SEPSIS: AN OVERVIEW
It is defined as the generalized inflammatory response elicited by an infectious process. Systemic inflammatory response syndrome(SIRS) is defined as the presence of two or more of the following: (1) temperature greater th...
Mucoadhesive: As Oral Controlled Gastroretentive Drug Delivery System
Mucoadhesion is a field of current interest in the design of drug delivery systems. Mucoadhesive drug delivery system prolong the residence time of the dosage form at the site of application or absorption and facilit...
The Opportunities and Challenges Involved in Registration of Similar Biotherapeutic Products in Emerging Countries
Although global biosimilars market looks attractive and continue to grow, the regulatory and operational hurdles remain in emerging regions. Many emerging nations are establishing biosimilars regulatory pathways, giving...